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Describe the difference between a single digest and a double digest?
Wiki User
∙ 10y ago
Restriction enzymes (endonucleases) are used for a variety of reasons in molecular genetics, including
obtaining a "map" and cloning DNA.
Single digests consitute DNA being treated with one restriction endonuclease, whereas double
digests contain 2 enzymes.
At times, it is difficult (or not possible) to perform double digests ... especially when the 2 enzymes have very different requirements for their activities (e.g. salt concentration, temperature optimums, ...).
If a DNA restriction map is known for a particular enzyme, and if the DNA is treated with this enzyme, then one can ascertain whether the digest was complete or not. However, if a restrictioin map is just being compiled, and if the DNA is treated with 2 enzymes in a double digest, at times difficulties may arise in determining the map if either (or both) enzymes did not completely digest the DNA.
Wiki User
∙ 10y ago
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