What is the Effect of dilution on enzyme activity?

Answer 1

At a high ion concentration, the ion interfere with the bonds between the side groups of the amino acids making up the enzyme (which is a protein). This causes the enzyme to lose its shape, called denaturation. If the enzyme loses its shape, it can no longer accept and react substrate, so the rate of enzyme activity decreases.

Answer 2

As the enzyme concentration increases the rate of enzyme activity increases up to a level where it becomes constant.

This is because the more the enzymes are available, the more substrates are broken in less time.

It then becomes constant as the substrate acts as a limiting factor, which means that there are not enough substrates to be broken down compared to the number of enzymes.

Answer 3

the greater the enzymes there are, the quicker the reaction rate, since there are more enzymes to act on the number of substrates.

i am not quiet sure about how enzyme activity, though. sorry.

Answer 4

As you increase the concentration of enzyme, the total activity will increase, but the activity per unit of enzyme will not change. That is, specific activity of the enzyme remains constant, but absolute activity in the mixture will increase, and more product will be produced per unit time as you increase enzyme concnentration.

Answer 5

Diluting either the substrate or the enzyme will result in a decrease in enzymatic activity, due to a reduction in the frequency of collisions (collision theory) between enzyme and substrate.

Answer 6

Temperature, pH, substrate concentration