You need more light at higher powers. The reason is simple; you're looking at a smaller field with less surface for light to fall on. Discounting the light lost in the optics, a field at 50X has four times the light of one at 100X.
Generally it decreases (with optical instruments, anyway).
increase
increase
microbiology Its limitation for higher magnification to observe tiny objects of viruses.
The two main advantages of SEM over light microscopy is that the SEM has a much deeper depth of field and that it has higher resolution than a light microscope can achieve and the resultant much higher magnification from the SEM.
Light.
Yes, electron microscopes have a much higher possible magnification than compound light microscopes. An electron microscope is capable of 10,000,000 times magnification, whereas a good compound light microscope is capable of 1,000 - 2,000 times magnification.
Magnification focuses light into a smaller area making it appear more intense.
It is more desirable to increase light when changing to a higher magnification using a compound microscope as the lens require more light. With proper lighting , it is easier to see specimen details as the lens aperture decreases with higher magnification.
The simplest optical microscope is the magnifying glass and is good to about ten times (10X) magnification. The compound microscope has two systems of lenses for greater magnification, 1) the ocular, or eyepiece lens that one looks into and 2) the objective lens, or the lens closest to the object.
as a piece of glass or other transparent material, used to converge or diverge transmitted light, to form optical images, as for magnification or correcting defects of vision
The amount of light needed increases as one moves to higher magnification with the microscope. This is usually done by opening the light diaphragm.
Higher Magnification
It's actually simple: The more the magnification, the smaller a "picture" of the available light you are getting - hence the more magnification, the darker the image looks. It works the same way with an astronomical telescope: The more magnification you use (assuming the objective lens doesn't change) the dimmer the object gets. To put it another way - there is a big difference between how high a magnification you are using, and how much light is reaching your eye.
A lens is a device that changes the direction of light.
you are viewing a smaller area so you need less light
The objective lenses are the optical element that gathers light from the 'object' being observed and they focus the light rays to produce a real image.
microbiology Its limitation for higher magnification to observe tiny objects of viruses.
The two main advantages of SEM over light microscopy is that the SEM has a much deeper depth of field and that it has higher resolution than a light microscope can achieve and the resultant much higher magnification from the SEM.
Light.