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What is transient transfection?
Transient transfection is a method used to introduce foreign DNA into cells for a temporary period, typically a few days. This allows researchers to study gene expression, protein function, and other cellular processes without integrating the foreign DNA into the host cell genome. Transient transfection is often used in laboratory settings for quick and reversible manipulation of gene expression.
What do you call pieces of DNA that are put together?
Engineered strain or mutant strain. If they are to be propagated in an organism, the mode of transformation/transfection is called a vector. Target DNA + vector = charged vector.
Why is PCR often used prior to cloning a gene in cells?
You need to make multiple copies of the gene. Almost no lab protocol is 100% effective, which includes ligation/recombination or transformation/transfection.
What has the author Walter Doerfler written?
Walter Doerfler has written: 'Foreign DNA in mammalian systems' -- subject- s -: Adenoviruses, DNA, Genetic transformation, Genetics, Mammals, Transfection 'Viren' 'Adenovirus DNA'
What method of plant transformation is used to introduce a gene in to chloroplast genome?
particle delivery system
When genes from one organism are placed into the cells of another organism what is this called?
That depends on what the other organism is. When DNA is inserted into a prokaryote (E. Coli for example), we call it transformation. When DNA is inserted into a eukaryoate (yeast or human cells for example), we call it transfection.
Could you insert DNA in cell?
yes, e.g. using a process called transfection.
What are the tools and processes that scientists use to manipulate human DNA?
The use of a vector and transfection is often used.
What practical use can the transfection process be used for?
The transfection process can be used practically in procedures where foreign DNA needs to be introduced into bacteria or mammalian cells. It can be used in investigating gene function and modulation of the gene expression, contributing to cellular research, drug discovery, and target validation.
What is the process in which plamid combines with a human gene?
Transfection is the process in which plamid conbines with a human gene.
What does transformation and competent and plasmid mean?
transformation is to introduce a foreign DNA segment to a competent cell. competent cell means a cell that have ability to take a foreign DNA. plasmid is genetic element that one can integrate a DNA segment. it has its promoter, resistance gene, multiple cloning site...
What are the factors that can affect the transfection efficiency and how?
seeding density of the cell quality of transfecting dna ratio of dna to transfectant reagent
