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To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
It is positive for the metabolizing of glucose.
yes it will ferment the mannitol and cause yellow color change.
To differentiate it from other gram positive, bacteria that are coccus.
It is urease positive .It possesses the enzymes urease which hydrolyses urea to form ammonia. As a result the pH of the medium used for detection of urease enzyme (Urea Broth) increases and shows bright pink colour, since phenol red is bright pink in alkaline conditions.
To diagnose Enterococcus faecalis: (Facultative anaerobic) First, do the Gram stain: Gram positive cocci in chains catalase test: negative PYR disc: positive
It is positive for the metabolizing of glucose.
yes it will ferment the mannitol and cause yellow color change.
To differentiate it from other gram positive, bacteria that are coccus.
can methyl-dopa make you test positive for barbituates
no no
no it is positive
Metabolic tests such as an oxidase test can be used to differentiate S epidermidis and E faecalis. Such a test can be conducted by adding H2O2 to the colony. The result for S aureus will be negative and the result for S epidermidis will be positive.
The morphologically similar Enterococcus (catalase negative) and Staphylococcus (catalase positive) can be differentiated using the catalase test.
Staphylococcus epidermidis indole test - negative methyl red - negative voges proskauer test - positive citrate test - no idea
Hi, Iodoform test is used for the detection of methyl ketones as methyl ketones give positive iodoform test. In this test, methyl ketone is treated with iodine (I2) in the presence of base such as sodium hydroxide (NaOH) to give iodoform(CHI3 - a light yellow coloured ppt). Chemistry of iodoform test is: Secondary alcohols also give positive iodoform test because during the reaction conditions, it gets oxidized to a methyl ketone.
Methyl red differs from Phenolred (which is used in the fermentation test and the MSA plates) in that it is yellow at pH 6.2 and above and red at pH 4.4 and below. Phenol red turns yellow below a pH of 6.8. If you get a positive with methyl red and a negative with phenol red, the pH has to be between 6.7 and 6.1.