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Freezing doesn't effect the enzymes since freezing does not permanently affect enzyme structure. Boiling permanently changes the structure and can change the enzymes.
Freezing temperature make ice of the water in bacteria and completely stop there metabolic activity. Enzymes do not work at freezing temperature as they have no scope for movement in solid ice. So there activity halted.
The reason why you should never hold a pineapple too long is because, pineapples contain very unique enzymes called BROMELAIN. These enzymes are pretty harmful because if you hold a pineapple too long, the pineapple enzymes will start to consume your skin. After a while you will have no finger prints because those enzymes would have eaten them away. Unless you want smooth fingers.
freezing provides unsuitable temperature for activities of enzymes
34 degrees Fahrenheit
yes
Substrate concentration will affect enzymes because substrates are specific to enzymes. The pH will affect enzymes because certain enzymes will work better in certain pH levels.
Because it keeps the enzymes and bacterias out of the food
Vinegar will not affect the freezing point of vinegar.
Yes, the enzymes in pineapple and papaya as well as citrus break down dead skin cells. They also break down food that is inside your body.
Pineapple contains an enzyme which prevents gelatin from setting. Heat destroys (denatures) enzymes.
Yes, pineapple left long on any meat will make it mushy. It is the acidic nature of pineapple.