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Cell seeding density refers the number of cells (usually per square centimeter) that are placed into a flask during cell passage. If growth is wanted a typical cell seeding density would be around 5,000 cells/square cm, however, if a high confluence is needed it may be more appropriate to seed cells around 50-60,000 cells/square cm.

How to determine cell seeding density-

look at the cells in a hemocytometer. This is a small plate that has very small squares etched into its surface, which allows for counting of individual cells underneath a microscope to determine the density of the cells in the cell suspension, and this the total number of cells can be approximated.

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Audra Weissnat

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2y ago
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12y ago

Yes, because each plant needs a certain amount of space in order for it to grow. If you plant them too close together, then the plants will croud each other and there will not be enough room for them to grow.

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13y ago

A pot has a limited soil volume, so the more seeds you plant in it, the more plants there are competing for the limited soil resources available. Overcrowded plants tend to be tall and skinny, because they're fighting with each other for light. They also flower early due to the stresses of competition, and produce smaller, inferior fruit or foliage. Overcrowded plants are also more susceptible to diseases, especially fungal diseases that thrive where air circulation is poor and plants are stressed.

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11y ago

As population density gets higher so does the importance of direct competition. The higher the competition the less plants will be able to grow at an optimum rate.

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15y ago

yes

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12y ago

not really

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Q: Does seed depth effect the plants growth?
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