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It can go either way.
no
Yes it should be hyphenated.
No, there is no hyphen between "non" and "fiction" in the word "nonfiction." The term is typically written as one word.
No. The term "biggest" has no hyphen. The slang compound adjective "biggest-ever" would.
The correct term is "ex-wife," with a hyphen.
Some say yes, you do need a hyphen because the term great is integral to the definition of great-grandmother. Otherwise, a great grandmother is just a grandmother who is terrific. Other people see no need and d not use hyphens for this.
I'd say real life uses a hyphen only when it is being used as a modifier as in, for example, real-life examples otherwise it shouldn't require one. Another possibility is to italicize the term real life to distinguish it for emphasis purposes since the term is somewhat redundant otherwise.
There is a hyphen in phrases like "100-year", because they precede whatever they are modifying, like a "100-year flood". In general, two-word phrases are hyphenated when they are used as adjectives and precede the term they modify.
double
The term is diploid.
This cannot be shown because it is not true. The 29th term will be double the 19th.