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From what i read in my book:

Because the capsule is nonionic, unlike the bacterial cell, the primary stain adheres to the capsule without binding to it. Since the capsule is water- soulube, copper sulfate, rather than water, is used to wash the purple primary stain out of the capsular material without removing the stain that is bound to the cell wall.

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Q: Explain the function of Copper Sulfate in the spore staining procedure?
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The copper sulfate is used as a decolorizing agent rather than water. The copper sulfate washes the purple primary stain out of the capsular material without removing the stain bound to the cell wall. At the same time, the decolorized capsule absorbs the copper sulfate, and the capsule will now appear white in contrast to the deep purple of the cell.


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Copper sulfate is not a metal There are two compounds called Copper Sulfate, which are salts of the metal Copper. CuSO4 is Copper (II) Sulfate, once known as Cupric Sulfate. Cu2SO4 is Copper (I) Sulfate, once known as Cuprous Sulfate.


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Copper sulfate has CuSO4 as its formula. Copper sulfate is also written copper (II) sulfate.


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Copper sulfate has CuSO4 as its formula. Copper sulfate is also written copper (II) sulfate.