Asked in BiologyMicrobiologyMicroscopes
Factors affecting Resolving power of microscope?
April 26, 2008 12:37PM
The limit of resolving power of a microscope is described by the Abbe criterion:
d being the minimal resolvable distance between two spots of the object
wl being the wavelength of the light used
NA being the numerical aperture of the microscope, which is equal to n*sin(a) with
n being the refraction index of the immersion liquid between object and objective
a being the aperture angle
because sin(a) is always smaller than 1 and n cannot rise above 1.7, the maximal resolving power of a microscope is about
and thus only depends on the wavelength of the light used, which normally will be about 600 nm.