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In which direction should you move the inoculating loop in the Bunsen burner flam?
When sterilizing a loop, grasp the handle firmly and begin flaming it starting at the end near the grip, flaming slowly down towards the loop, being sure that the wire is glowing orange. This ensures that the loop is being flamed properly and sterilizing.
Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
How can prevent splattering when flaming a loop which has just used to transfer a culture?
you should drawn the loop slowly from the cooler to the hotter part of flame.Heat from the base of the wire first and slowly move towards the loop's tip. Heat the wire until it is red-hot.
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
Would a false positive from the reaction between the inoculating loop and hydrogen peroxide be caused by poor specificity or poor sensitivity of the catalase test system?
The false positive from the reaction of hydrogen peroxide and the inoculating loop would be caused by poor specificity. The formula for specificity is TN/TN+FP.
In which direction should you move the inoculating loop in the Bunsen burner flam?
When sterilizing a loop, grasp the handle firmly and begin flaming it starting at the end near the grip, flaming slowly down towards the loop, being sure that the wire is glowing orange. This ensures that the loop is being flamed properly and sterilizing.
What is the purpose of flaming the loop before and after use?
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
The small amount of growth on the end of an inoculating loop is called an?
Inoculum
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
In which direction should you move the inoculating loop in the Bunsen burner flame?
Move the inoculating loop in a back-and-forth motion through the top part of the blue cone of the Bunsen burner flame. This ensures that the loop gets heated evenly and reaches the optimal temperature for sterilization.
Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
Why should you let the inoculating loop cool first before and after using it?
The inoculating tube is cooled before use so that the organism is not killed by extensive heat.
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
How can prevent splattering when flaming a loop which has just used to transfer a culture?
you should drawn the loop slowly from the cooler to the hotter part of flame.Heat from the base of the wire first and slowly move towards the loop's tip. Heat the wire until it is red-hot.
What is the most commonly used instrument used for transferring cultures from a broth?
inoculating loop and needle.
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample from an agar plate?
Air contamination is prevented by sterilizing the inoculating loop with a Bunsen burner flame before and after use. This kills any potential airborne bacteria that could contaminate the sample. It's also important to keep the agar plate closed as much as possible while transferring the sample to further reduce the risk of contamination.
