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There is no "set" time limit. For proper aseptic technique, you should wait until the inoculating loop/needle is visibly red so that all organisms are dead, etc.
Wiki User
∙ 14y ago
Wiki User
∙ 11y ago3 to 4 seconds, in the hottest part of the flame on a Bunsen burner
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In which direction should you move the inoculating loop in the Bunsen burner flam?
When sterilizing a loop, grasp the handle firmly and begin flaming it starting at the end near the grip, flaming slowly down towards the loop, being sure that the wire is glowing orange. This ensures that the loop is being flamed properly and sterilizing.
Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
Why flaming the loop when streaking for isolation?
To sterilize it.
Function of inoculator loop?
Inoculating loops are for the transferring of microorganisms or for the staining of slides. ?æIt is also called a smear loop or a micro streaker.?æ
How do you prevent splattering when flamming a loop which has just been used to transfer a culture?
By sterilizing the loop by flaming it.
What is the purpose of flaming the loop before and after use?
Inoculating loop is used to inoculate microbial colony or sample on culture medium and to avoid the undesired microbial cells or to avoid contamination flaming of inoculating loop is necessary it is also called as incerination.
In which direction should you move the inoculating loop in the Bunsen burner flam?
When sterilizing a loop, grasp the handle firmly and begin flaming it starting at the end near the grip, flaming slowly down towards the loop, being sure that the wire is glowing orange. This ensures that the loop is being flamed properly and sterilizing.
How is air contamination prevented when an inoculating loop is used to introduce or take a bacterial sample from an agar plate?
The plate cover is raised and held diagonally to protect the plate surface from any contamination in the air.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
The small amount of growth on the end of an inoculating loop is called an?
Inoculum
Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
Why should you let the inoculating loop cool first before and after using it?
The inoculating tube is cooled before use so that the organism is not killed by extensive heat.
Why flaming the loop when streaking for isolation?
To sterilize it.
How inoculating needle use?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
How would you make sure a inoculating loop was sterile?
Pass the loop through a flame 2 or 3 times for about 10 seconds at a time.
What is the most commonly used instrument used for transferring cultures from a broth?
inoculating loop and needle.
