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Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What else can I help you with?
What is an inoculating needle used for?
An inoculating needle is used in a microbiology lab to collect microorganisms from a culture and move them to a petri dish.
Inoculating an agar deep tube?
inoculation needle
Why should use an inoculating needle when making smears from a solid media?
You should use an inoculating needle when making smears from solid media because of the solid's density. The smaller areas are denser, so it is easier to retrieve these specimens using an inoculating needle.
What are other inoculating instruments aside from needle?
Air gun and inoculating loop are some of the another inoculating instruments. The air gun is used to squirt thin stream of vaccine.
Why did you use an inoculating loop instead of a needle to make the transfers from the culture plates to the culture tubes?
A inoculating loop is used for transfers from culture plates to culture tubes instead of the inoculating needle because the needle could puncture the agar in tube. The loop is much easier as well to get liquid amount into the tube.
What is an inoculation?
Inoculating needle is used like a pen. Hold it like you hold a pen. Inoculating loop and a needle is mainly used to pick a single colony(pure) so u need to be gentle on the agar. practice using an inoculating needle on a paper with pen.
What is the most commonly used instrument used for transferring cultures from a broth?
inoculating loop and needle.
Why must a straight inoculating needle be used when inoculating an agar deep tube?
This is important to prevent the inoculating needle from becoming stuck in the agar, taking out pieces of agar while trying to remove the instrument. This agar will get into the inoculum when sterilizing the needle on the flame, causing contamination to your sample.
Why should you use an inoculating needle when making smears form solid media?
You should use an inoculation needle for making smears from a solid media so you can control how much specimen is put onto a slide. This method is easier for solid media and you would use a loop for liquid media.
Why do you use an inoculating loop instead of a needle to make the transfer from the culture plates to the culture tubes?
Because the solid media is more dense over a smaller area so a inoculating needle is used to retrieve the specimen. Where as for a liquid medium the specimen is more spread out over the liquid. The inoculating loop can collect more liquid because there is more metal present at the inoculating specimen retrieval point and has the ability to collect liquid in the loop. I'm currently taking general microbiology and my lab book hardly covers this. A.C.
When inoculating agar deep stabs why do you have to insert and remove the needle along the same stab line?
To minimize the introduction of unwanted oxygen into the medium.
Why does a straight inoculating needle used to inoculate an agar deep tube?
because you want to introduces as little air as possible
