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liquid nitrogen (-170 C) which it freeze the tissue to become fragile to be a fine powder which increase the surface area of extraction, and the very low temperature prevent DNase activation
* the primary function of this step is to lyse the cells
Wiki User
∙ 11y ago
Wiki User
∙ 9y agoLiquid nitrogen is often used in the cell disruption portion of DNA extraction. Tissue is quickly frozen with liquid nitrogen and then ground into a powder. This allows cells to be broken open.
Wiki User
∙ 10y agoIt freezes the cell, easy to break cell wall, finer powder produced, higher yield of DNA.
It also keeps harmful chemicals and natural enzymes (e.g. nucleases) deactivated due to its very low temperature.
Wiki User
∙ 12y ago1.) used to freeze the plant tissue and blend it to powder.
2.) minimize DNA degradation when grinding samples
Wiki User
∙ 13y agoCheap, easy, fast, and food tastes fresher.
Wiki User
∙ 12y agoliquid nitrogen is employed to break down cell wall material and allow access
to DNA while harmful cellular enzymes and chemicals remain inactivated
Wiki User
∙ 7y agoIt is cheap, plentiful, non toxic, EXTREMELY cold and fairly easy to transport.
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What is the function of dish washing liquid in onion DNA extraction?
It cleans and moisturizes without leaving streaks.
What is the function of 2-propanol in DNA extraction?
To achieve precipitation DNA.
What is the function of dithiothreitol in DNA extraction?
DTT is the reducing agent for thiolated DNA
What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
What is function of Buffer AP1 in DNA extraction?
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
What is the function of dish washing liquid in onion DNA extraction?
It cleans and moisturizes without leaving streaks.
What is the function of 2-propanol in DNA extraction?
To achieve precipitation DNA.
Function TE in extraction DNA?
chelating Mg2+
What is the function of dithiothreitol in DNA extraction?
DTT is the reducing agent for thiolated DNA
What is the function of chloroform in DNA extraction?
It solubalize lipids and protiens to remove them from DNA
What is the function of liquid detergent in genomic DNA extraction?
Liquid detergent used in the genomic DNA extraction, emulsify plasma membrane and nuclear membrane promoting lysis. SDS (Sodium Dodecyl Sulphate) is an anionic detergent used in DNA extraction. It removes the positive ions from the proteins, due to this protein loses its conformation and gets destroyed thus the cell membrane gets damaged and cell gets broken.
What is the function of CL buffer in DNA extraction?
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
What is the function of ascorbic acid in DNA extraction?
removal of polyphenols
What is the point of mashing straberrys for DNA extraction?
Use the usual extraction protocol for plants: grind, liquid-liquid extraction, salting out.
What is the function of ammonium acetate in DNA extraction?
ammonium acetae use to percipitate DNA from water.
Function chloroform in extraction DNA from blood?
to remove excess phenol from DNA to remove excess phenol from DNA
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
