DTT is the reducing agent for thiolated DNA
To achieve precipitation DNA.
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.
Maintaining the osmotic pressure to prevent the cell form bursting.
It cleans and moisturizes without leaving streaks.
To achieve precipitation DNA.
chelating Mg2+
It solubalize lipids and protiens to remove them from DNA
In a DNA extraction, the purpose of a buffer is to solubilize DNA as well as RNA. Because of this, it prevents the DNA for degrading.
removal of polyphenols
ammonium acetae use to percipitate DNA from water.
to remove excess phenol from DNA to remove excess phenol from DNA
Triton X-100 is used as a lysis buffer for DNA separation.
salt binds to molecules to keep it from clumping
The buffer AP1 is vital in DNA extraction as it acts as a cleanser to break up the lipids surrounding the cellular membrane. The buffer also maintains the right environment for the DNA so it is not damaged during the extraction process.
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
to extract the DNA from the solution, isopropyl alcohol is mixed with the DNA solution to precipitate the same.