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It dissolves the cell mebrane.

Keep in mind that some people do not use Triton X-100 in cell lysis, but instead sonificate...

Also Triton X-100 can be replaced by a whole lot of other stuff (CHAPS, Igepal, etc)

Hope to have givin you enough info

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Q: Function of Triton X-100 in Lysis buffer?
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How does Triton X 100 effect cells?

Triton X-100 is a non-ionic detergent that can disrupt cell membranes by solubilizing lipids and proteins. It can lyse cells by disrupting the lipid bilayer and breaking down membrane structures, leading to cell death. It is commonly used in cell lysis protocols to release cellular contents for downstream analysis.


What is the difference between Triton X100 and CHAPS detergent?

Triton X-100 is a nonionic detergent commonly used to solubilize membrane proteins, while CHAPS is a zwitterionic detergent that is milder and often preferred for protein solubilization due to its lower denaturation potential. CHAPS is also less likely to interfere with downstream applications such as enzyme assays or chromatography compared to Triton X-100.


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