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Triton-X100 permeabilizes cell membranes, and allows reagents access to both the cytosol and nuclear material. Triton-X100 is often used to stain fixed cells with antibodies, especially for BrdU or DAPI type staining.
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How do you make 0.3 percent Triton X-100?
triton x-100 stock percent? 100% ?
How many body cells does a body have?
An adult human has approximately 75-100 X 1012 cells. (75,000,000,000,000 to 100,000,000,000,000 cells)
What sort of chemical would be the most effective for breaking down cell membranes?
detergents like Triton-X or SDS
What is the difference between Triton X100 and CHAPS detergent?
There are multiple differences. Triton X-100 is non-ionic . It has both hydrophilic and hydrophobic regions, but no net charges. CHAPS is zwiterionic. It has hydrophobic regions but also a head group with a negative charge (in normal saline). In addition, Triton X100 forms large (greater than 90,000 MW) aggregates when TGriton X100 concentration rises above 0.25 mM. CHAP on the other hand forms smaller aggregates (6,000 MW) when CHAPS concentration rises above 10 mM.
What sex chromosomes does a female have in all her cells?
A female human has two X sex chromosomes in her cells. A male human has one X and one Y sex chromosome in his cells.
How do you make 0.3 percent Triton X-100?
triton x-100 stock percent? 100% ?
How to make 0.1 percent Triton X-100?
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers.
Role of Triton x-100 in DNA extraction?
it iS A ionic detergent disrupts bonds between lipids and fosters the isolation of DNA....
Function of Triton X-100 in Lysis buffer?
It dissolves the cell mebrane. Keep in mind that some people do not use Triton X-100 in cell lysis, but instead sonificate... Also Triton X-100 can be replaced by a whole lot of other stuff (CHAPS, Igepal, etc) Hope to have givin you enough info
What is the function of tritonx100 in DNA extraction?
Triton X-100 is used as a lysis buffer for DNA separation.
What is an 10 x 10 grid 1-100?
A 10 x 10 grid would contain 100 cells.
How many body cells does a body have?
An adult human has approximately 75-100 X 1012 cells. (75,000,000,000,000 to 100,000,000,000,000 cells)
What is 100 to the 6th power?
1006 = 100 x 100 x 100 x 100 x 100 x 100 = 1000000000000
What digits multiplied equals 100?
1 x 100 = 100 2 x 50 = 100 4 x 25 = 100 5 x 20 = 100 10 x 10 = 100 20 x 5 = 100 25 x 4 = 100 50 x 2 = 100 100 x 1 = 100
What is x out of 100 to equal 78 percent?
x = 78 Equation: x/100 = 78% Solution: x/100 = 78% x/100 = 0.78 x = 0.78 * 100 x = 78
All the factor pairs of 100?
100 = 1 x 100 100 = 2 x 50 100 = 4 x 25 100 = 5 x 20 100 = 10 x 10
5 multiples of 100?
1 x 100 = 1002 x 100 = 2003 x 100 = 3004 x 100 = 4005 x 100 = 500
