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iodine act as a mordant..on the gram positive bacteria which got really thick and abundence of peptidoglycan layer, the crystal violet will fix to the peptidoglycan layer..meanwhile in gram negative bacteria which is lack of peptidoglycan layer, the alcohol or acetone will wash it away
What else can I help you with?
What is the function of tincture iodine in trichrome stain?
Tincture iodine in trichrome stain is used as a mordant to enhance the staining of collagen fibers. It helps to improve the contrast and visibility of collagen in the tissue sample.
What function of lugol's iodine in gram staining?
fixing the stain so that the first dye which is the crystal violet will not be washed away during rinse process.
Is iodine a basic stain?
No, iodine is not a basic stain. Iodine is commonly used in Gram staining to identify bacteria as either Gram-positive or Gram-negative based on their cell wall composition. It acts as a mordant in the staining process and helps to fix the crystal violet stain in Gram staining.
Why iodine oinment called non-staining?
Iodine ointment is called non-staining because it does not leave a permanent mark or discoloration on surfaces or fabrics it comes into contact with. This makes it easier to use without worrying about staining clothes or other items.
Why is iodine important to your world?
iodine is used for disinfecting water while camping and staining specimens for microscope use
Differences between lugols iodine and grams iodine?
Lugol's Iodine = 20g Potassium Iodide + 10g Iodine dissolved in 1L H2O Gram's Iodine = 6.7g potassium iodide + 3.3g Iodine dissolved in 1L H2O Recipe's taken from Flinn Scientific Catalog I have on campus. Looks to me as Gram's is just a dilute version of Lugol's. Same ratio of KI:I just less of it. Couldn't tell you much about differences in application however.
What structure does iodine stain?
Iodine stains starch molecules blue-black. This is commonly used in biological staining techniques to visualize starch content in cells or tissues.
What is the function for Iodine?
The function of iodine is to keep good working of thyroid gland and the throat.
Can food coloring be used to substitute methylene blue in staining cheek cells?
iodine
Why use iodine in plant cells staining?
Iodine is used in plant cell staining to highlight the presence of starch. Iodine forms a complex with starch molecules, turning them blue or black, making them easily visible under a microscope. This staining method is commonly used to identify starch storage sites within plant cells.
Why is Iodine used in Gram staining?
Iodine is used in Gram staining as a mordant, which helps to bind the crystal violet dye to the cell wall of bacteria. This mordant-iodine complex forms larger complexes with the crystal violet dye, making it difficult for the dye to be washed away during the decolorization step. This allows for differentiation of Gram-positive and Gram-negative bacteria based on their ability to retain the crystal violet dye.
What is the difference between strong iodine solution and weak iodine solution?
their color
