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Gram staining is primarily used to differential bacteria based on their cell wall structure. Bacteria are usually classified as Gram positive or Gram negative.
Gram positive - thick layer of peptidoglycan as outermost layer, plasma membrane as innermost layer.
Gram negative - thin layer of peptidoglycan "sandwiched" in between 2 separate plasma membranes.
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∙ 12y ago
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∙ 12y agoGram staining is a common technique used to differentiate two large groups of bacteria based on their different cell wall constituents. The Gram stain procedure distinguishes between Gram positive and Gram negative groups by coloring these cells red or violet. Gram positive bacteria stain violet due to the presence of a thick layer of peptidoglycan in their cell walls, which retains the crystal violet these cells are stained with. Alternatively, Gram negative bacteria stain red, which is attributed to a thinner peptidoglycan wall, which does not retain the crystal violet during the decoloring process.
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∙ 14y agoGram staining is done to determine
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∙ 11y agobacteria
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Can simple staining techniques be used to identify more than the morphological characteristics of microorganisms?
Yes, you can determine the arrangement of the micoorganism such as chain, random, or spiral as the simple staining allows you to obtained a distinctive contrast between background and its organisms being observed.
What color does the gram positive stain?
It will hold the primary stain of violet.
Would it be useful to perform a gram stain on a mixed culture?
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
Purpose of decolorizing agent?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pink color.
How could you tell if one of the colonies on the plate was a contaminant that fell in from the air?
If one of the colonies was to be contaminated by something in air, you would be able to tell by there being a difference in look for specimen. Also looking to see if something is growing on top of smooth specimen.
What test would be useful to differentiate between the two genera if you see Gram positive cocci under the microscope but you cant quite tell the arrangement of the cells?
Gram staining would be the test that would be useful to differentiate between the two genera if you see gram positive cocci under the microscope, especially if you can't quite tell the arrangement. Another name for the gram staining test is Gram's method.
What is the value of gram staining?
Gram staining is highly valuable. It allows us to identify two widely different bacteria. Gram staining can tell you if the bacteria is pathogenic or if a penicillin pill can cure it. It tells us gram-positive bacteria, or gram-negative. Positive being easily combated bacteria and some even helpful, and gram-negative being primarily pathogenic.
Grams method is used to tell what?
Gram staining is primarily used to differential bacteria based on their cell wall structure. Bacteria are usually classified as Gram positive or Gram negative. Gram positive - thick layer of peptidoglycan as outermost layer, plasma membrane as innermost layer. Gram negative - thin layer of peptidoglycan "sandwiched" in between 2 separate plasma membranes.
What value is the gram stain?
Gram staining is highly valuable. It allows us to identify two widely different bacteria. Gram staining can tell you if the bacteria is pathogenic or if a penicillin pill can cure it. It tells us gram-positive bacteria, or gram-negative. Positive being easily combated bacteria and some even helpful, and gram-negative being primarily pathogenic.
Can simple staining techniques be used to identify more than the morphological characteristics of microorganisms?
Yes, you can determine the arrangement of the micoorganism such as chain, random, or spiral as the simple staining allows you to obtained a distinctive contrast between background and its organisms being observed.
What color does the gram positive stain?
It will hold the primary stain of violet.
How can you differentiate gram positive and gram negative bacteria?
The process of gram staining is simple. 1)smear bacteria from pure culture onto slide, heat fix 2)flood with crystal violet (1min) 3)Add iodine (1 min) 4)acid/alcohol wash (1 min) 5)Flood with safranine (1min) 6)Air dry and examine. These times are for clinical microbiology and experimental methods employ optimal and more precise times (but overall its pretty close). Down side of this method is that you must smear bacteria onto the slide and fix it by heating the underside of the slide with a bunsen burner. if they are pink then you have gram negative (Gram's stain didnt stick) if its purple then its gram positive(Gram's stain did stick) This is due to the peptidoglycan layers. Gram negative bacteria have only a thin layer of peptidoglycan as part of the cell membrane/wall where Gram positive have a very think peptidoglycan layer. Source(s): Medical Microbiology
Can you tell an organisms gram stain by simple staining?
Gram stain is not a simple stain because simple stains do not use two or more stains. Gram stain is a differential stain differentiating between Gram positive (blue-black) and Gram negative (pink-red).
Would it be useful to perform a gram stain on a mixed culture?
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
What is relative atomic mass and how it is related with gram?
when atomic mass of an element expressed in gram atom is called gram atom mass . and formula is number of gram atom=given mass (in gram) by atomic mass ( in gram )
How do you convert micro gram into milligram?
if you are willing to have a poo on the girl in America called Chrisy i Will be willing to tell you poo face
How can you tell if giardia is gram negative or positive?
I don't believe that G. lamblia is either one. That is it cannot be identified via gram staining. A trichrome stain is needed to identify this bacteria. The trichome stain, originally a system of three colored dyes, illuminates the nuclei in the parasite and along with it's unique shape (that of a tear drop with two nuclei) identifies the parasite as giardia.
