No so far there hasn't been a episode where Rukia has been tickled.
Yes. Everyone has been tickled in the belly button.
Most people get tickled at least a few times in their life, so I'm quite sure the Jonas Brothers have been tickled more than once.
No.
She has probably been tickled a few times. She a cute laugh.
Yes
no.well,not that i know of.
In Sonic x episode 2, Chuck (thinking Sonic was a robot) tickled him.
I'm not sure, but Ash probably got tickled by Misty a few times.
yes he has
It is believed by many scientist that yes, he did.
I don't believe so.