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How do you kill RNA?
RNA can be degraded by Ribonucleases (aka RNases). These are enzymes found all over the inside and outside of your body and are made by all living things. RNA isn't alive, so it is not being killed; it is being fragmented into small pieces.
What is superasin?
An RNAse inhibitor, made by Ambion http://www.ambion.com/techlib/resources/superasein/
What enzymes are in the mouth?
The saliva in mouth has following enzymes:salivary amylase or ptyalin (breaks down carbahydrates)lingual lipase (enzyme for fats digestion)lysozyme (enzyme that kills bacterial cell)salivary ribonuclease (RNase)Deoxyribonuclease (DNase)
Why 1M of HCL is used in Dnase test?
what is the role of HCL in the DNase experiment
Is serratia marcescens dnase positive or negative?
Serratia genus as a whole is dnase positive.
What is the purpose of RNA extraction?
RNAse destroys the RNA and hence RNAse contamination is a problem in RNA extraction as it breaks down RNA. RNAse enzyme is removed by using RNAse inhibitor or precautions like wearing of gloves, autoclaving tips , using RNAse free water/DEPC treated water is done while performing RTPCR
How can you tell if isolated RNA is free of DNA?
There is a DNA killing step in RNA isolation by the enzyme DNase I. This will make sure your preparation is free of DNA.
Role of RNase in DNA purification?
RNAase remove RNA from the solution as we need DNA only. RNAse cuts down the RNA strand.
What is the reagent used to test Dnase test?
There are no reagents added when a Dnase test is performed. The test is done in a methyl green medium with a pure inoculum culture. If halos form around the culture than Dnase is present.
Does DNase affect RNA?
nope.
Role of EDTA in DNA isolation?
it is chealeting agent and has great affinity with metal ions and mg- ions present in dnase as a cofactor and responsible for dnase action that degreded DNA hear edta bide with mg- ions and stop the action of dnase.
What is DNAse?
DNase (deoxyribonuclease) is an enzyme. It is manufactured by ribosomes and can undergo post translational modifications or cotranslational modifications. DNase catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. A wide variety of is known, which differ in their substrate specificities, chemical mechanisms, and biological functions.
