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Which of the three main steps of pcr is responsible for the specificity in the small piece of DNA that gets amplified by replication?
I hate hole's class too!
What is the defference between Real-time PCR and reverse transcriptase PCR?
Difference between real time PCR and reverse transcription PCR is as follows:- 1. Real time PCR is donated as qPCR and on the other hand reverse transcription PCR is denoted as RT-PCR. 2. In qPCR, the template used is single strand DNA strand whereas in the RT-PCR, the template used in process is single strand of RNA. 3. The real time PCR enables both quantification as well as detection of the DNA in the real time whereas the RT-PCR enables only the quantification of the RNA and it is little bit slower process then the qPCR as it first produce the cDNA from the template RNA strand and then process it in the similar fashion as the traditional PCR.
Advantages and disadvantages of PCR techniques?
Some advantages are that it can create many copies of a minimal starting amount ie good for criminal investigations if you find some hair you can use PCR to find out whose it is. Also another advantage is PCR is speedy you can make tons of copies in hours. Disadvantages are that with PCR cannot substitute for gene cloning in cells when large amounts of a gene are desired. Also occasional errors during PCR replication impose limits on the number of good copies that can be made. source Campbell and Reece Biology 7th ed
Why do you use a negative control in PCR?
to check is there any contamination in pcr products
What is the function of EDTA in PCR?
It Inhibits the PCR reaction by chelating the magnesium ions.
What natural process is PCR like?
Replication.
Which molecular technique involves DNA replication in a tube?
Polymerase Chain Reaction (PCR) involves DNA replication in a tube
How many rounds of replication of pcr before the first discrete products are formed?
The First discrete PCR product will be found in the 3rd round.
How does PCR selectively amplify stretches of DNA?
The PCR or Polymerase Chain Reaction is a laboratory system for DNA replication and amplificiation. It allows selected stretches of DNA to be duplicated using heat in the process.
What feature of a DNA molecule ensures accurate replication of the strands during each PCR cycle?
Complementary base pairing
If you have a small gene that you wish replicated by pcr you add radioactively labeled nucleotides to the pcr machine after 3 replication cycles how many double-stranded DNA molecules do you have?
2x2x2=8 stranded DNA molecules
Which of the three main steps of pcr is responsible for the specificity in the small piece of DNA that gets amplified by replication?
I hate hole's class too!
Compare the polymerase chain reaction and DNA replication?
PCR need thermostable enzyme like taq DNA polymearse, while in replication using highly proofreading enzyme DNA polymerase. taq enzyme work in very high temprature while in replication our body temprature
What transcription similar to in the process of DNA?
replication
How are individual DNA strands are constructed?
DNA replication duplicates the DNA strands. This process is accompanied by various enzymes such as DNA polymerase, Helicase, Topoisomerase.In labs they are constructred by Polymerase chain reaction(PCR).
What are the different types of polymerase chain reaction techniques?
types of pcr: AFLP -PCR. Allele-specific PCR. Alu-PCR. Assembly -PCR. Assemetric -PCR. Colony -PCR. Helicase dependent amplification. Hot start pCR. Inverse -PCR. Insitu -pCR. ISSR-PCR. RT-PCR(REVERSE TARNSCRIPTASE). REAL TIME -PCR
What is the difference between replication of DNA and amplifcation of DNA in PCR?
DNA cloning is where you take a piece of DNA and put it in a host cell so that every time the host cell replicates, its daughter cells will have that exact copy of DNA. DNA amplification is just taking a piece of DNA and making copies of it, like in the process of PCR. it is not inside a host cell. another way to think of it: you can amplify a gene--make a bunch of copies of it, and then clone it (by putting it in a cell and once that cell replicates each daughter cell has a copy of that DNA). you don't need to amplify anymore in cloning, you already did that before.
