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DNA replication and PCR are related in that they both involve the process of copying DNA. DNA replication occurs naturally in cells to create new copies of DNA for cell division, while PCR is a laboratory technique that amplifies specific DNA sequences by replicating them in a controlled environment.
What else can I help you with?
What is the process by which a DNA molecule is copied called?
The process by which a DNA molecule is copied is called DNA replication. During DNA replication, the two strands of the double helix separate and each strand serves as a template for the synthesis of a new complementary strand. This results in two identical copies of the DNA molecule.
How do DNA bases pair up in the process of DNA replication?
During DNA replication, the DNA bases pair up in a specific way: adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). This pairing is essential for accurately copying the genetic information stored in DNA.
What is PCR?
Taq are important in PCR because they are heat resistant and are required for polymerase action. Hence we use Taq Polymerase enzyme in PCR. Specially and their main role is "Heat Sensitive Polymerase Enzyme".
Why does a new DNA strand elongate only in the 5' to 3' direction during DNA replication?
During DNA replication, a new DNA strand elongates only in the 5' to 3' direction because DNA polymerase can only add nucleotides to the 3' end of the growing strand. This is due to the structure of the DNA molecule and the way the nucleotides are arranged.
Why does a new DNA strand elongate only in the 5' to 3' direction during replication?
During DNA replication, a new DNA strand elongates only in the 5' to 3' direction because DNA polymerase can only add nucleotides to the 3' end of the growing strand. This is due to the structure of the DNA molecule and the way the nucleotides are arranged.
What is the difference between replication of DNA and amplifcation of DNA in PCR?
DNA cloning is where you take a piece of DNA and put it in a host cell so that every time the host cell replicates, its daughter cells will have that exact copy of DNA. DNA amplification is just taking a piece of DNA and making copies of it, like in the process of PCR. it is not inside a host cell. another way to think of it: you can amplify a gene--make a bunch of copies of it, and then clone it (by putting it in a cell and once that cell replicates each daughter cell has a copy of that DNA). you don't need to amplify anymore in cloning, you already did that before.
Is there an easy way to explain the polymerase chain reaction?
The Polymerase Chain Reaction (PCR) results in the rapid production of many copies of a DNA sequence. PCR makes it possible to study DNA fragments which have DNA that are too small or damaged to allow other types tests.
What is the process by which a DNA molecule is copied called?
The process by which a DNA molecule is copied is called DNA replication. During DNA replication, the two strands of the double helix separate and each strand serves as a template for the synthesis of a new complementary strand. This results in two identical copies of the DNA molecule.
How do DNA bases pair up in the process of DNA replication?
During DNA replication, the DNA bases pair up in a specific way: adenine (A) pairs with thymine (T), and cytosine (C) pairs with guanine (G). This pairing is essential for accurately copying the genetic information stored in DNA.
What is PCR?
Taq are important in PCR because they are heat resistant and are required for polymerase action. Hence we use Taq Polymerase enzyme in PCR. Specially and their main role is "Heat Sensitive Polymerase Enzyme".
Why does a new DNA strand elongate only in the 5' to 3' direction during DNA replication?
During DNA replication, a new DNA strand elongates only in the 5' to 3' direction because DNA polymerase can only add nucleotides to the 3' end of the growing strand. This is due to the structure of the DNA molecule and the way the nucleotides are arranged.
Why does a new DNA strand elongate only in the 5' to 3' direction during replication?
During DNA replication, a new DNA strand elongates only in the 5' to 3' direction because DNA polymerase can only add nucleotides to the 3' end of the growing strand. This is due to the structure of the DNA molecule and the way the nucleotides are arranged.
How does a PCR machine work to amplify and detect specific DNA sequences?
A PCR machine works by repeatedly heating and cooling a sample containing DNA. This process, called thermal cycling, allows specific DNA sequences to be copied, or amplified, many times. The machine also includes a detector that can identify the presence of the amplified DNA sequences, providing a way to detect and analyze specific genetic material.
Why are there many replication forks during DNA replication?
The DNA polymerase enzymes can only operate from the 3' end of the chain to the 5' end, not the other way. The two helices that make up the double helix of DNA are oriented oppositely, as shown in the diagram above, making the DNA polymerase enzymes move in opposite directions.
Why was polymerase chain reaction developed?
makes more copies of a sample of DNA. apex
What is the type of replication of DNA that occurs in laboratory vessel?
I'm not entirely clear about what you're asking. Labs do a lot of things with DNA. However, generally DNA is made in most laboratories more or less the same way DNA is made in cells: using an enzyme called a DNA polymerase which uses one strand of DNA as a template to make a second strand from free dNTPs. This type of replication I suppose would be called in vitro replication or, more commonly PCR (polymerase chain reaction) which is no doubt explained elsewhere on this site. For large scale DNA replication, I believe the standard protocol is still bacterial transformation. The DNA of interest is enclosed in a ring of DNA, called a vector, capable of being carried within bacteria. The vector is then inserted into chemically treated bacteria and then the bacteria are allowed to grow in culture overnight. As the bacteria multiply and divide, they also copy the vector DNA and your DNA of interest, increasing the amount of vector DNA by severl orders of magnitude. After culturing a large volume of bacteria, you can then lyse the cells and purify out the DNA you wanted to replicate. DNA can also be synthesized from modified bases chemically, but this generally can only make relatively small strands of DNA.
Explain the function of replication?
DNA replication requires the opening of the 'zipped up' DNA strand. This is so a 'new' strand of DNA can be inserted and have a template strand to 'read' off. DNA polymerase analyses the bases on the template strand and adds each complementary base to synthesise the 'new' strand. In order for DNA polymerase to be able to do this the DNA has to be opened up by helicase to reveal the bases of the template strand. The unzipping of the DNA by helicase forms the replication fork. Thus the function of the replication fork is to reveal template strands for DNA replication to actually occur.
