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How are ribosomes recycled?

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ConnerMiskowiec

Lvl 1
10y ago
Updated: 8/21/2019
After translation stops and the polypeptide chain has been released, the ribosome needs to dissociate off of the mRNA transcript. A Ribosome Recycling Factor (RRF) will insert itself into the A-pocket of the ribosome (this A-pocket still contains a stop codon). Elongation Factor G (EF-G) then binds to the RRF. This binding stimulates the release of the two tRNAs that are in the P and E pockets of the ribosome. The RRF and the EF-G are then displaced from the ribosome. The mRNA is also released and the two halves of the ribosome are allowed to dissociate.
Afterwards, the small ribosomal subunit is either bound by IF1 an IF3 or elF1 and eLF1A depending on the organism being a prokaryote or eukaryote. These proteins block tRNAs from entering into its E and A pockets.
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