The iris diaphragm controls the amount of light passing through the slide or specimen, while the substage condenser focuses a cone of light on the slice or specimen.
It should be kept half the way between the body and the base.
Parts of the light Microscope 1. Ocular lens or eyepiece: most are 10x magnification. The scopes used are binocular (two eyepieces). 2. Body tube: contains mirrors and prisms which direct the image to the ocular lenses. 3. Nosepiece: holds the objective lenses, rotates 4. Objective lenses: usually 3-4 on our scopes, 4x, 10x, 43x, 100x oil immersion (red banding). Total magnification = ocular power x objective power. Most of our binocs have fixed position lenses--the stage moves up and down rather then the lens. 5. Stage: Movable platform on which slides are mounted for viewing; all of the scopes have mechanical stages with X,Y vernier scales. Focus knobs move the stage up and down. 6. Condensor: A substage lens which focus the light on the specimen. The binocs have condensors that move up and down to focus the light beam. 7. Iris Diaphragm: the diaphragm is located just below the stage and controls the amount of light which passes to the specimen and can drastically affect the focus of the image. 8. Focusing knobs: outermost is the fine focus and innermost is the coarse focus. On the binocs these knobs control up/down movement of the stage. 9. Light source: The scopes have built in light sources. The rheostat ON/OFF switch is located either on the scope or on the external power supply and is used to regulate light intensity.
The substage condenser on a microscope gathers the light from the microscope's light source. It distributes the light evenly over the entire view field.
The substage condenser is a part of the light microscope that that serves to concentrate light from the source and focus it through the object and magnify it by the objective lens.
The iris diaphragm controls the amount of light passing through the slide or specimen, while the substage condenser focuses a cone of light on the slice or specimen.
The iris diaphragm controls the amount of light passing through the slide or specimen, while the substage condenser focuses a cone of light on the slice or specimen.
Condenser height is controlled by a rack and pinion gear system that allows the condenser focus to be adjusted for proper illumination of the specimen.
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substage needs a condenser, and passes light through the tissue section from below. Epi-illumination reflects light off of the specimen from behind
It is where you adjust the light comming from the mirror.
Your light intensity knob which is either on the right, or left of the base of the microscope. When you increase your magnification via the objective, increase your substage iris diaphragm. ie) 10x objective ~ 0.2, 40x ~ 0.4
It should be kept half the way between the body and the base.
The cast of Substage - 2012 includes: Elodie Lavoignat as Ballerina
The condenserA) is located below the substage light B) should be as close to the stage as possible C) moves the stage to the right or left D) contains an additional lens for viewing