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how artificial chromosome are used as cloning vectors with example?

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Q: How artificial chromosomes are used as cloning vectors with example?
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Is a cloning vector a plasmid?

plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.


What are the types of vectors are there in biotechnology?

there are two types of vectors cloning vector and expression vectors.


What is the difference between cloning and expression vectors?

Cloning vectors are used to increase the number of copies of the cloned gene or to amplify a foreign gene. Expression vectors are used to increase the expression of the foreign gene product.


What is the insert capacity of PACs vectors in DNA cloning?

In a M13 phage we can insert about 1-4 kb of target DNA sequence which is its maximum insert capacity.


What are the roles of cloning vectors?

cloning vectors are the carriers of a particular characteristic. it can be inserted in the non coding region of the DNA and then replicated to yield a modified population.for example; production of insulin from e.coli bacteria. in its non coding sequence human insulin producing gene is inserted and it is allowed to multiplicate, as a result we get a population of the bacteria producing insulin.


What are two types of vector used in recombinant DNA technology?

there are many different vectores as: 1-plasmid system 2-bacteria phage lamda 3-cosmids 4-bacterio artificial system 5-puc system the other cloning vectors are m13 which is the oldest one. and after the above all are:- BAC(bacterial artificial chromosome) YAC(yeat artificial chromosome) TAC(transformation-competent artificial chromosome)


What are phage vectors?

"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).


Transfer devices called cloning are used to overcome problems in getting DNA into cells?

Vectors


Explain the role cloning vectors play in making recombinant DNA?

they carry a gene like a vehicle


How are genes transfered?

gene transferring methods includes: gene gun method, biopsy, electroporation, biolistics, cloning vectors.


What does plasmids do?

plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.


What has the author P H Pouwels written?

P. H. Pouwels has written: 'Cloning Vectors, A Laboratory Manual. Supplementary Update 1987'