how artificial chromosome are used as cloning vectors with example?
plasmid is the type of the cloning vector. other cloning vectors includes cosmids, bacteriophage, phagemids, artifiical chromosomes. clonong vectors are the carriers of certain traits to be inserted in non coding regions of the DNA.
there are two types of vectors cloning vector and expression vectors.
Cloning vectors are used to increase the number of copies of the cloned gene or to amplify a foreign gene. Expression vectors are used to increase the expression of the foreign gene product.
In a M13 phage we can insert about 1-4 kb of target DNA sequence which is its maximum insert capacity.
cloning vectors are the carriers of a particular characteristic. it can be inserted in the non coding region of the DNA and then replicated to yield a modified population.for example; production of insulin from e.coli bacteria. in its non coding sequence human insulin producing gene is inserted and it is allowed to multiplicate, as a result we get a population of the bacteria producing insulin.
there are many different vectores as: 1-plasmid system 2-bacteria phage lamda 3-cosmids 4-bacterio artificial system 5-puc system the other cloning vectors are m13 which is the oldest one. and after the above all are:- BAC(bacterial artificial chromosome) YAC(yeat artificial chromosome) TAC(transformation-competent artificial chromosome)
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".Commonly used vectors include plasmid, Lambda phage, cosmid and yeast artificial chromosome (YAC).
Vectors
they carry a gene like a vehicle
gene transferring methods includes: gene gun method, biopsy, electroporation, biolistics, cloning vectors.
plasmids are vectors that are used to replicate recombinant DNA in a host organism or cell. they are normally used in cloning experiments.
P. H. Pouwels has written: 'Cloning Vectors, A Laboratory Manual. Supplementary Update 1987'