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there are many different vectores as:
1-plasmid system
2-bacteria phage lamda
3-cosmids
4-bacterio artificial system
5-puc system
the other cloning vectors are
m13 which is the oldest one.
and after the above all are:-
BAC(bacterial artificial chromosome)
YAC(yeat artificial chromosome)
TAC(transformation-competent artificial chromosome)
What else can I help you with?
What is the methodology for producing recombinant DNA to be used in gene cloning?
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
What is a vector used for in a genetic engineering experiment?
"Vector" is an agent that can carry a DNA fragment into a host cell. If it is used for reproducing the DNA fragment, it is called a "cloning vector". If it is used for expressing certain gene in the DNA fragment, it is called an "expression vector".
What types of ic chips are there?
Thousands, if not millions. There are so many that it would be a major task to catalog them all. They could be categorized by technology used or by function.
What are the different kinds of technology?
TECHNOLOGY actually means 'knowing how to do something'. Anything you know how to do is a technology.In modern times there are a number of different types of technologies emerging; these are centred around ways we live and how we make our lives more comfortable. For example:1. Assistive technology is technology used by individuals with disabilities in order to perform functions that might otherwise be difficult or impossible2. Medical technology refers to the diagnostic or therapeutic application of science and technology to improve the management of health conditions3. Information technology refers to both the hardware and software that are used to store information4. Communications technology5. Transport technology6. Defence technology
What are the steps of using recombinant DNA technology?
1 Isolate DNA 2 Cut DNA with a restriction enzyme 3 Mix the DNA's and join then together by using DNA ligase 4 Insert the recombinant plasmid into a host bacterium 5 Allow the bacterium to reproduce
What is a small double stranded DNA that's used as a vector?
it is a ds DNA use in recombinant DNA technology to insert our interested gene and multiply it.Ex;plasmid,cosmid
Significance of vector in DNA recombinant technology?
Vector are plasmid DNA, act as a molecular vehicles to carry genes or DNA of interest. In rDNA technology vectors used to clone the gene by ligation. This chimeric DNA or plasmid can be propagated in E.coli as the vector carries its own origin of replication. Expression plasmid vectors can be used to produce proteins from the gene of interest.
What is puc vectors?
A pUC vector a circular, double stranded DNA molecule normally used for recombinant protein expression. It is not a math vector.
What technique is used to make bacteria that produce drugs?
recombinant technology
What are the types of vectors are there in biotechnology?
In biotechnology, vectors can include plasmids, bacteriophages, and viral vectors. These vectors are used to transfer genetic material into host cells for various applications such as gene cloning, gene therapy, and protein production. Plasmids are commonly used in recombinant DNA technology, while viral vectors are often used in gene therapy.
When a biological vector is used which process must take place before recombinant DNA can be placed in the host cell?
The biological vector must first be transformed with the recombinant DNA using a suitable method such as heat shock or electroporation. This process involves introducing the recombinant DNA into the vector so that it can carry and deliver the genetic material into the host cell.
What is pcDNA?
pcDNA is DNA vector used to clone recombinant DNA sequences for the expression of proteins of interest in mammalian cells.
How can recombinant DNA technology be used to combat HIV infections?
Recombinant DNA technology can be used to develop antiretroviral drugs that target specific components of the HIV virus to inhibit its replication. It can also be used to produce vaccines that induce immune responses against HIV. Moreover, gene therapy approaches using recombinant DNA can be used to modify immune cells to make them resistant to HIV infection.
What is the Genetic engineering technique used for creating monoclonal antibody?
recombinant DNA technology
What was the experiment used for cloning?
Recombinant DNA technology was used with a sheep like animal named dolly.
Explain how DNA technology transfer bacterial genes from cell to cell?
I think you must rethink about your question, but still I am giving the answer as I can understand that you are asking about recombinant DNA technology where bacterial DNA is used as it is a cloning vector (plasmid). In recombinant DNA technology the particular sequence of DNA that we want to replicate or want to produce in huge number, is attached either with plasmid of bacteria or a DNA of bacteriophage and thus produce the recombinant or hybrid DNA which is copied each time when the bacteria or bacteriophage multiply. In this way the hybrid DNA will be transferred from parent cell to daughter cells.
Explain the role of klenow fragment in recombinant DNA technology?
The Klenow fragment, derived from the DNA polymerase I enzyme, is used in recombinant DNA technology to fill in the single-stranded DNA gaps left in a vector after annealing with a DNA insert. It possesses 5' to 3' polymerase activity and 3' to 5' exonuclease activity, allowing it to extend the DNA strands in a template-directed manner. This helps to create recombinant DNA molecules with high efficiency.
