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Anarobes can be inoculated in an agar slant using an inoculating needle. The needle is able to put the anarobes deep inside the agar. The anarobes can then grow in the anarobic environment provided. The agar slant should then be refrigerated after suitable growth is achieved.

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13y ago
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14y ago

For long period culture can be preserved in glycirene solun (20%) in MCTs or you can preserved them by making slant culture in testtubes, in both the above method low temp is used to kept the cultures. You can also use lyophilizer which is an advanced way to preserve culture, in this all the water content of culture is soaked by lyophilizer machine and culture with dry material is preserved in vaccum enviornmetn.

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Q: How can anaerobes be maintained in pure cultures?
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Bacteria that only grows with the absence of oxygen?

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Do obligate anaerobes grow on a slant if they are incubated anaerobically?


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Anaerobes is associated with methanogens.


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Anaerobes cannot be cultivated in the presence of atmospheric oxygen.


Can streak or spread plates isolate pure cultures from mixed bacteria?

YES


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Obligate anaerobes do not need or use oxygen as a nutrient. In fact it is a toxic substance to them which either kills or inhibits their growth. Thioglycollate allows obligate anaerobes to grow because it consists of sodium thioglycollate, thioglycollic acid and L-crystine which reduce oxygen to water. This creates an anaerobic environment allowing obligate anaerobes to grow.


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