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Q: How can one determine the purity of isolated DNA sample?
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What is the principle behind Quantification of DNA by reading its optical density at 260nm and 280nm?

It is to check the purity of the DNA sample where it may have the interaction of RNA and sheared DNA in it.


How do you anylyze DNA?

I guess you need to state your answer more precisely. What is it what you like to analyse with the DNA sample you have in your hands (guessing you isolated some DNA before; there are several methods for doing so). Here are some examples what I am analysing: * Measure the amount / DNA-concentration (Absorption of UV-light) * Measure the length of the DNA (electrophoresis) * Determine the sequence


How do you find whether given sample is DNA or RNA?

A spectrophotometer can be used to know if a sample is DNA or RNA. DNA has an absorbance maximaat 260nm, whereas RNA has an absorbance maxima at 280nm. By looking at which one of these two wavelengths the sample is more excited, one can determine if the sample is DNA or RNA.


What happens if your DNA is taken?

Not sure what you mean by this question... If a sample of your DNA is taken, it can be compared to some other sample of tissue to determine if that other tissue sample came from your body. It can also be used to determine paternity/maternity, or how similar your DNA is to someone elses. It could also potentially be used to determine your genetic predisposition for certain heritable characteristics, such as various diseases or even personality traits.


What can one do with a DNA kit?

With a DNA kit you can use the swabs and a sample of your saliva to see if someone's DNA matches another person. This is often used to determine paternity of a child.


What process could a what forensic scientist use to analyze a DNA sample from a crime scene to identify a criminal?

A forensic scientist would use a method like PCR (Polymerase Chain Reaction) to amplify the DNA sample, creating more copies for analysis. They would then use DNA sequencing techniques to compare the sample to a suspect's DNA profile or a DNA database to identify a match. This process helps determine the source of the DNA sample and link a suspect to the crime scene.


Why DNA isolated stored in buffer not water?

So as to maintain a favourable pH for the isolated DNA


How will we know after DNA isolation if the material is DNA or not or how will we identified the DNA in DNA isolation proceess?

check the absorption of the extracted material at 260nm... After DNA isolation, there is the possibility of protein contamination. If there are small changes in the way the isolation is done and the amount of detergents added and the centrifugation speeds, they could affect the final purity of the isolated DNA. Another common contaminant is RNA. Once the DNA has been purified, a small amount of the sample is taken for spectrophotometric analysis. Here, the sample is exposed to light of 260 and 280nm wavelength and the absorbency is noted. The ratio of the absorbency at these two wavelengths is calculated. If the ratio is between 1.8 and 2.0, then the DNA is considered pure for further applications. If not, then the isolation protocol has to be changed or the reagents have to be replaced in toder to obtain pure DNA


When was DNA found?

DNA was first isolated in 1869.


What is the difference between using DNA profiles to determine paternity and to match a tissue sample with a suspect?

DNA profiles are shorter and determine whether the suspect was involved immediately, they can also use a very small amount of evidence, such as a small drop of blood or a single hair to match a DNA fingerprint. A tissue sample requires more evidence from the scene as well as the suspect in order to match them.


What is used to create a large sample of DNA for testing for a small sample of DNA?

PCR


Does DNA contian sugars?

DNA is the result of the y and x chromosome combining in the womb. DNA can be taken from anywhere to determine the 3 trillion letters that make up who you are. So unless the part of the body that the DNA sample is taken from produces sugars, then no, DNA does not contain sugar.