How are Petri dishes sterilized?

Answer 1

To determine that an agar plate is sterlize you can keep it overnight in incubator without streaking and if there is any growth peresent in it then it is contaminated and if not then it is obveiously sterlize

Answer 2

Petri dishes are usually sterilised by the manufacturer (check package). You can check it by adding media (like LB broth or agar) to one or two plates of the batch and leave them overnight at 37 degrees Celsius. If nothing grows, consider them sterile.

When in doubt, autoclave them and always autoclave agar before poring plates.

Answer 3

Agar is a medium, so you are checking the sterility of the agar. After preparation one usually places an agar plate at room temperature and another agar plate at 35 to 37 degrees C. After 24-48 hrs of incubation a visual check is made to see if there is any visible growth on the uninoculated plates.

If you are adding blood, etc. to the agar, those components can be checked by subbing them to a blood agar plate to see if there is any growth--which would indicate non-sterile components.

Answer 4

With detergents