0
When the gene is multiplied with PCR it's possible to add specific sequences on either side. These sequences are recognised by restriction enzymes. Some restriction enzymes leave a "sticky end", where one strand of DNA is a bit longer than the other. If you select the sticky ends correctly you can direct which end of the gene will ligate (stick) to which end of your vector.
What else can I help you with?
If you place gene in reverse orientation how can it be detected?
Most genes contain restrictions sites. Once you've inserted your gene into the vector you use restrictions sites in the gene and the vector to cut the vector into smaller pieces. If the pieces correspond to the pattern you expect for a reverse orientated gene then you know it is in the reverse orientation.
What was the sexual orientation of gene autry?
There was no evidence that Gene Autry was anything but straight.
What are chromosomal changes which involve gene-orientation but do not result in gene expression changes called?
inversions
Why has the gay gene not disappeared from the human gene pool?
Sexual orientation is complex and likely influenced by multiple factors, both genetic and environmental. There is no single "gay gene," but rather a combination of genetic and environmental factors that may contribute to sexual orientation. Additionally, diversity in sexual orientation may confer evolutionary advantages, such as increased social cohesion within groups.
What happen to the magnetic orientation of rocks as the poles reverse?
There is nothing -_-
What happens to the magnetic orientation of rocks as the pole reverse?
There is nothing -_-
Reverse the poles produced in a coil?
To reverse the poles produced in a coil, you can simply reverse the direction of the current flow through the coil. This will change the magnetic field orientation and reverse the poles.
What happens to the magnetic orientation of rocks as the poles reverse?
During a magnetic pole reversal, the magnetic orientation of rocks changes to align with the new orientation of the Earth's magnetic field. This means that the magnetization of rocks will also reverse during a geomagnetic field reversal event.
What is it called when a fragment of a chromosome breaks off and then reattaches to the original chromosome at the same place but in the reverse direction?
This process is called an inversion mutation. It involves a fragment of a chromosome breaking off, flipping, and rejoining at the same location but in the reverse orientation. Inversions can have different effects on gene expression and can sometimes lead to genetic disorders.
What are mutations in which a piece of code breaks flips and reattaches backwards?
Inversions are mutations where a segment of code breaks off, flips, and then reattaches in the reverse orientation within the genome. This can lead to changes in gene expression and potentially impact the function of the affected genes.
What was the sexual orientation of Gene Anthony Ray?
Gene Anthony Ray lived in a time when there was still a lot of stigma regarding sexual orientation. He never publicly discussed it. All we know is that he never married, and that he was HIV positive at the time of his death in 2003.
Does an isometry preserves orientation?
An isometry is a transformation that preserves distances between points, and it can either preserve or reverse orientation. For example, a rotation is an isometry that preserves orientation, while a reflection is an isometry that reverses orientation. Therefore, whether an isometry preserves orientation depends on the specific type of transformation being applied.
