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Most genes contain restrictions sites. Once you've inserted your gene into the vector you use restrictions sites in the gene and the vector to cut the vector into smaller pieces. If the pieces correspond to the pattern you expect for a reverse orientated gene then you know it is in the reverse orientation.

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Q: If you place gene in reverse orientation how can it be detected?
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Related questions

What was the sexual orientation of gene autry?

There was no evidence that Gene Autry was anything but straight.


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