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What is the ampr gene?
The ampr gene encodes for the enzyme beta-lactamase, which confers resistance to ampicillin in bacteria. This gene is often used as a selectable marker in molecular biology experiments to identify transformed cells that have taken up a plasmid with the gene.
What is the role of gene tagging in gene analysis?
Gene tagging is a method where a specific marker or reporter gene is added to a target gene in order to track its expression or function in cells or organisms. This technique allows researchers to study the behavior and location of the tagged gene, aiding in the analysis of gene expression, protein localization, and interactions within biological systems.
What gene makes it possible to identify bacteria that carry a plasmid?
The gene commonly used to identify bacteria carrying a plasmid is the beta-lactamase gene, which confers resistance to beta-lactam antibiotics. Bacteria harboring plasmids with this gene can be identified by growing them on agar plates containing beta-lactam antibiotics and observing which colonies survive.
A gene for antibiotic resistance may be used as a making it possible to identify a transformed cell?
Yes, a gene for antibiotic resistance can be used as a selectable marker in transformation experiments. By incorporating the gene into a vector along with the gene of interest, researchers can grow the transformed cells on media containing the antibiotic, allowing only the cells that have successfully incorporated the gene of interest to survive. This method helps in identifying and isolating the transformed cells.
Why do transformed cells form white colonies?
Assuming you are asking about blue-white screening in transformation of plasmids... The agar plate has X-gal in it. If a colony of E. coli has beta-galactosidase (an enzyme expressed from the lac operon in the vector) present, it will break down the x-gal and turn the colony blue. If the colony does not express beta-galactosidase (because the LacZ gene has been interrupted by a ligated gene that you want to express), it will not metabolize the x-gal, thus not turning blue.
What can gene for antibiotic resistance be used for?
genetic marker
A gene for antibiotic resistance may be used as?
Genetic Marker
What is maker?
Marker may be a gene having unique character in an organism.
How are cells screened to determine if the recombinant DNA was taken up?
Usually recombinant DNA is packaged in a plasmid that contains a marker gene. This marker can be an antibiotic resistance gene (NPTII for Kanamycin) or a gene that enables the plant to synthesise an amino acid. For antibiotic resistance the cells are grown on a medium that contains the antibiotic. The ones that grow have the marker gene. Sometimes the cells are transformed with a mixture of plasmids, some with the target gene and some without. The LAC-operon is used to select the cells that have the gene inserted. The gene-insertion inactivates the LAC-Z gene. Cells grown on X-gal plates will be blue, unless there's a transgene present. So white colonies have the transgene.
How can one detect polymorphism by genetic marker?
One can detect polymorphism by genetic marker using single-nucleotide polymorphism which is able to even tell mutation of a gene.
A gene that makes it possible to distinguish bacteria that carry the plasmid from those that dont?
Genetic marker.
What is the literary device for tic tic tic tic?
Onomatopoeia is the name of the literary device in which sounds are written into words.
Company that makes tic-tac?
tic-tac is a company that makes tic-tac
What is a tic?
A tic is an involuntary convulsion of muscles.
What is the ampr gene?
The ampr gene encodes for the enzyme beta-lactamase, which confers resistance to ampicillin in bacteria. This gene is often used as a selectable marker in molecular biology experiments to identify transformed cells that have taken up a plasmid with the gene.
Do they make lemonade tic tacs?
No they do not make lemonade tic-tacs but they do make lemon tic-tacs.
What is the role of gene tagging in gene analysis?
Gene tagging is a method where a specific marker or reporter gene is added to a target gene in order to track its expression or function in cells or organisms. This technique allows researchers to study the behavior and location of the tagged gene, aiding in the analysis of gene expression, protein localization, and interactions within biological systems.
