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How could you test that the media provided for this experiment were sterile?
Wiki User
∙ 6y ago
In the short term, you can see if there is any growth or if there is any change to the container. Otherwise you must do the media and sterilize it yourself or you must trust the person who did.
Wiki User
∙ 8y ago
Wiki User
∙ 13y agoIt depends. Is it already autoclaved (sterilised)? If so, you can prepare a nutrient or blood agar plate and spread plate out 100ul (if your media is a broth) onto the surface and incubate overnight to see if anything grows. If it's an agar plate, just incubate that overnight (usually at 37degrees). If anything grows, its not sterile.
Wiki User
∙ 12y agoOn a petri dish? If anything is growing on the media it definitely isn't sterile. It should be sealed.
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What is the Functions of filtration unit in animal tissue culture?
Most often, the media used for animal tissue culture has to be filtered before growing cells in them. Media contains components like serum and other growth factor proteins. Therefore, the media cannot be sterilized through heat. Sterile filtration is a widely used method of obtaining sterile media
Why is a sterile swab used to collect bacteria in the mouth?
because it is important that in nutrient media to grow up only bacteria that may be present in your mouth.In tha case tha swabs are not sterile in nutrient media will growth mouth and swab bacteria and the will give a flas positive result.
Importance of flaming the edges of the petri plates before and after pouring the media?
It's part of proper sterile procedures as a precaution against contamination of the media by microorganisms that have attached to the edge of the petri dishes since the last sterilization.
What is aseptic media fill?
its a process of simulatiing the actual manufactuirng process for sterile prodcuts. In this,the filling material is replaced by using suitable placebo (Lactose,PEG) along with micribological medium.
Why heat the inoculating loop after each transfer of microorganisms?
It is necessary to heat the inoculating loop after each transfer in order not to cross contaminate the media you are working with. It is also needed to maintain universal sterile procedures in a laboratory environment.
How could you tell that the media that was provided was sterile?
When in doubt, make your own. Or do one blank and other as usual. If you blanks grows something, then that whole batch of media was contaminated.
How can you tell that the media provided for this exercise were sterile?
Try to grow a plate with nothing on it. If something grows, it's not sterile.
Why should contrast media be labeled on the sterile back table in surgery?
yes, it should.i sell euiptment including sterile back tables and there is nothing wrong with that.
What is the Functions of filtration unit in animal tissue culture?
Most often, the media used for animal tissue culture has to be filtered before growing cells in them. Media contains components like serum and other growth factor proteins. Therefore, the media cannot be sterilized through heat. Sterile filtration is a widely used method of obtaining sterile media
Why is a sterile swab used to collect bacteria in the mouth?
because it is important that in nutrient media to grow up only bacteria that may be present in your mouth.In tha case tha swabs are not sterile in nutrient media will growth mouth and swab bacteria and the will give a flas positive result.
Which form of otitis media involves a buildup of pus in the middle ear?
If the middle ear, which is normally sterile, becomes contaminated with bacteria or viruses, pus and pressure in the middle ear can result, and this is called acute otitis media or suppurative otitis media.
How has the new media provided more privacy to adolescents today?
It decidedly has not.
Importance of flaming the edges of the petri plates before and after pouring the media?
It's part of proper sterile procedures as a precaution against contamination of the media by microorganisms that have attached to the edge of the petri dishes since the last sterilization.
How can you use media efficiently?
If you are careful using media it could lead to social media burnout.
What kind of services were provided by Google Friend Connect?
The services provided by Friend Connect were finding friends who are using on form of social media by accessing email or other forms of social media to see if friends have accounts. It is no longer in service.
What is YEMA media and why it is used in experiment?
YEMA is Yeast Extract Mannitol Agar. It is usually used for isolation of rhizobium.
What type of media are sterilized in the autoclave?
PBS can be safely autoclaved. It only contains inorganic salts, which are quite heat-stable. AFAIK media can not be autoclaved, because there are many different (organic) compounds in them, which would be lost because of the heating. One way you can save money, is to prepare media from powder, sterile filter large amounts (like 20 l or so) with one bottletop filter and store in 1 l sterile bottles. You have to add (sterile) glutamine and FBS (and whatever else you want) when you open the bottle.
