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Chemical equaton of reaction between glycine and ninhydrin solution?
nee amma puku
Why you will spray ninhydrin for analysis in chromatography?
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
Result of casein in ninhydrin test?
Kharkov's
What functional group must be present for the ninhydrin test to work?
Ninhydrin works because it reacts with the amino acids left behind in a latent print. Ninhydrin has a polar carbonyl carbon with is electron deficient. It is attacked by the nucleophilic nitrogren on an amino acid, temporarily combining the ninhydrin and amino acid molecule. The structure is rearranged until the origionally attacked carbon is protonated and leaves in the form of water. This creates a schiff base when the nitrogen is double bonded to the origionally attacked carbon. This molecule rearranges again so that the nitrogen is double bonded to the adjacent carbon of the amino acid. This last rearrangement produces carbon dioxide gas. Further rearrangement of the product produces ruheman's purple.
What has no effect on the rate of an enzyme-catalyzed reaction?
When an enzyme is saturated the amount of substrate added no longer as an effect on the rate of the reaction.
What is reaction of organolithium compound with dimethylformamide?
The product is an aldehyde.
What is ruhemanns purple formed in ninhydrin reaction?
It is the final coloured compound.
Chemical equaton of reaction between glycine and ninhydrin solution?
nee amma puku
What questions have been raised about ninhydrin?
Ninhydrin is a chemical used to expose latent fingerprints, but its use is declining because of the slow reaction time it takes for it to react with the oil in the fingerprints. You can use heat to speed the process up but there are other faster options.
Is ninhydrin the same as super glue?
NO, ninhydrin is not the same as super glue
Does ninhydrin has flaws?
Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.
Why proline amino acid gives yellow colour in reaction with ninhydrin?
Yes. Within "The Chemical Reactions of Amino Acids / Reactions of Amino Groups" there are several examples - one of which is the ninhydrin reaction. A very widely applied reaction of the alpha amino group [that is the N in the peptide bond -CCN-CCN-], it is used to estimate the quantity of amino acids [in a sample] in very small amounts. All amino acids and polypeptides with a free alpha group react with ninhydrin and yield [or produce] an intensely purple colored product - except for Proline and Hydroxyproline {both in which the alpha amino group is termed to be 'substituted' - something to do with carbon rings} which "yield derivatives with a characteristic yellow color." See also 'Schiff's bases'.
Is Dimethylformamide DMF suitable for UV spectroscopy detection?
Yes
What does DMF stand for?
1) Dimethylformamide 2) Drug Master File
What is the molecular formula of ninhydrin?
Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4
Can you get a reaction from morphine?
Of course you get a reaction. A reaction means that you experience some effect from the drug and you certainly will experience the effect of morphine!
Why you will spray ninhydrin for analysis in chromatography?
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
