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Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
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Why it is necessary to spray the chromatography with ninhydrin?
because it is a locating agent that identify the different substances , espically the invisible amine acid
What is isothermal analysis?
analysis of a mixture through chromatography :)
Can chromatography be used to separate mixtures?
Chromatography is used in case of an investigation as in the analysis of poisoned food, chromatography can help find individual components.
What does LC-MS mean in toxicology?
LC-MS is the abbreviation for Liquid Chromatography - Mass Spectrometry. It is a method for doing analysis.
In Gas Chromatography isothermal is when the temperature is constant but what is it called when the temperature is programmed to increase?
Temperature Programming (from what i learned in Quantitative Analysis)
Why it is necessary to spray the chromatography with ninhydrin?
because it is a locating agent that identify the different substances , espically the invisible amine acid
What is the spraying reagent used for citric acid paper chromatography?
ninhydrin reagent
What is isothermal analysis?
analysis of a mixture through chromatography :)
Why is ninhydrin used in amino analysis in TLC?
Ninhydrin is used in amino acid TLC because it readily stains amino acids by reacting with the amine groups. This reaction takes place very quickly and creates a brownish-color that can be easily visualized.
What has the author James F Lawrence written?
James F. Lawrence has written: 'Trace Analysis. Volume 1' 'High-performance liquid chromatography of pesticides' -- subject(s): Analysis, Analytic Chemistry, Chromatographic analysis, High performance liquid chromatography, Liquid chromatography, Pesticides
Can chromatography be used to separate mixtures?
Chromatography is used in case of an investigation as in the analysis of poisoned food, chromatography can help find individual components.
What has the author Douglas T Gjerde written?
Douglas T. Gjerde has written: 'Ion chromatography' -- subject(s): Ion exchange chromatography 'DNA chromatography' -- subject(s): Analysis, Chromatographic analysis, DNA, Genetic Techniques, High Pressure Liquid Chromatography, Isolation & purification
When the amino acids are colourless how will you get to know that which acid is this?
It really depends on the type of chromatography. E.g. with thin layer chromatography, UV light is used, and the amount of light that is absorbed is measured, and they can tell the amino acid from this. In paper chromatography, a substance called ninhydrin is sprayed onto the separated amino acids and they become visible.
What has the author Seaton T Preston written?
Seaton T. Preston has written: 'A guide to the analysis of phenols by gas chromatography' -- subject(s): Analysis, Gas chromatography, Handbooks, manuals, Phenols
What has the author Howard Purnell written?
Howard Purnell has written: 'Gas chromatography' -- subject(s): Gas chromatography, Chromatographic analysis
What has the author Monika Waksmundzka-Hajnos written?
Monika Waksmundzka-Hajnos has written: 'High performance liquid chromatography in phytochemical analysis' -- subject(s): High performance liquid chromatography, Phytochemicals, Analysis
Why is it necessary to spray the chromatogram with resorcinol?
Resorcinol is a colouring agent for some chromatography experiments.
