because it is a locating agent that identify the different substances , espically the invisible amine acid
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
These are the amino acids that will yield positive result to the ninhydrin test: Non-polar amino acids: Glycine, Alanine, Valine, Leucine, Isoleucine, Phenylalanine, Methionine, Tryptophan Polar Neutral Amino acids: Serine, Cysteine, Threonine, Asparagine, Glutamine, Tyrosine Polar Acidic Amino Acids: Aspartic acid and Glutamic acid Polar Basic Amino Acids: Histidine, Lysine, Arginine
Kharkov's
there are different types like gas chrom. and thinlayer chrom Answer: There are two types of chromatography:liquid chromatography gas chromatography
What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography? What is the precedure for calibration of gas chromatography? How calibration is perform for gas chromatography?
Since amino acids are colourless compounds, ninhydrin is used for detecting them. To identify this, after development, the TLC plate is sprayed with ninhydrin reagent and dried in an oven, at 105°C for about 5 minutes. Ninhydrin reacts with α- amino acids that results in purple coloured spots [(due to the formation of the complex - Rheuman's purple).
ninhydrin reagent
Resorcinol is a colouring agent for some chromatography experiments.
It really depends on the type of chromatography. E.g. with thin layer chromatography, UV light is used, and the amount of light that is absorbed is measured, and they can tell the amino acid from this. In paper chromatography, a substance called ninhydrin is sprayed onto the separated amino acids and they become visible.
Ninhydrin is used in amino acid TLC because it readily stains amino acids by reacting with the amine groups. This reaction takes place very quickly and creates a brownish-color that can be easily visualized.
NO, ninhydrin is not the same as super glue
Yes ninhydrin does have flaws. The most common flaw of ninhydrin is the fact that it is so sensitive to the extent of not recording a fingerprint if enough perspiration wasn't created.
It help to separate and analyze colorless substances in chromatography by reacting with the colorless substance to form colored spots on the chromatogram.
These are the amino acids that will yield positive result to the ninhydrin test: Non-polar amino acids: Glycine, Alanine, Valine, Leucine, Isoleucine, Phenylalanine, Methionine, Tryptophan Polar Neutral Amino acids: Serine, Cysteine, Threonine, Asparagine, Glutamine, Tyrosine Polar Acidic Amino Acids: Aspartic acid and Glutamic acid Polar Basic Amino Acids: Histidine, Lysine, Arginine
Well, the best I could come up with is it's either:C9H6O2orC4H3O2If someone knows any different please correct me.jman63: it is actually C9H6O4
Kharkov's
No! because the Ninhydrin test is mostly used to detect ammonia or primary secondary amines