When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.
to fix the color of the stain
Benedicts reagent is used to check reducing sugars , it need hat to work and gives red color when positive ,. While biuret reagent is for proteins .there is no need to use heat and gives purple , violet color when positive ..
When the isolated beta amylase enzyme is subjected to the biuret test, you will observe a color change from yellow to blue. This is because the biuret reagent reacts with the peptide bonds in the enzyme, causing a change in color.
sugars are converted to enediols by benedict's reagent on boiling. these enediols reduce Cu(II) to Cu(I) which then forms CuOH (yellow in color). on heating CuOH yields Cu2O which is orange/red in color.
The indicators which change color is litmus paper strips.
Indol Test ( Kovac's Reagent): E.a. = negative = yellow/gold ring at meniscus; as for E.c. = positive = red ring at meniscus (immediate change). Methyl Red Test (Methyl Red Reagent): E.a. = negative = yellow = pH > 7.0; as for E.c. = red color = pH < 5 (immediate change). Vogues-Proskauer Test (Napthol + KOH Reagent): E.a. = positive = reddish color (not immediate change); as for E.c. = negative = brown/yellow (straw color). Citrate (Bromothymol Blue Reagent - alkaline pH): E.a. = positive = blue color + growth; as for E.c. = negative = green color.
store in original container at room temperature
When being tested with biuret reagent, protein turns a blue to lilac color. However, starches when tested do change to a blackish blue color.
blue
The glucometer will need to be calibrated with each box of reagent strips. Because each manufacturer does it differently, please refer to the owners manual for accurate instructions.
Albustix strips typically change colors from yellow to green to blue in the presence of protein in urine. This color change corresponds to different levels of protein concentration in the urine sample.
blue
to fix the color of the stain
Benedicts reagent is used to check reducing sugars , it need hat to work and gives red color when positive ,. While biuret reagent is for proteins .there is no need to use heat and gives purple , violet color when positive ..
The reagent that is used to test for starch is a mixture of iodine and potassium iodide in water, or an Iodine - KI reagent. If the reagent turns blue-black in color, then starch is present.
black