Indol Test ( Kovac's Reagent): E.a. = negative = yellow/gold ring at meniscus; as for E.c. = positive = red ring at meniscus (immediate change).
Methyl Red Test (Methyl Red Reagent): E.a. = negative = yellow = pH > 7.0; as for E.c. = red color = pH < 5 (immediate change).
Vogues-Proskauer Test (Napthol + KOH Reagent): E.a. = positive = reddish color (not immediate change); as for E.c. = negative = brown/yellow (straw color).
Citrate (Bromothymol Blue Reagent - alkaline pH): E.a. = positive = blue color + growth; as for E.c. = negative = green color.
I - +ve M - +ve VP - -ve C - -ve
The IMViC series of tests provides an identification of the principal groups of Enterobacteriaceae that cause intestinal infections, by using the biochemical properties and their enzymatic reactions to specific substrates
I recently completed colony morphology on the E.colispecimen.The results displayed small colonies that were circular in shape, a diameter of approximately 0.5mm, the elevation of the colonies was seen to be slightly raised with the surface incredibly smooth.The colonies were also seen to have a pink colour pigmentation in Mac conkey agar due to its lactose fermenter after growth.The gram stain for these bacteria should be negative rod shaped.
The Kirby-Bauer antibiotic test is a general test that determines if an organism is resistant or susceptible to different antibiotics. Many different types of antibiotics can be used but some of the common ones are penicillin, tetracycline, chloramphenicol, and streptomycin.
IMVIC farmula for e.coli is ++--
I - +ve M - +ve VP - -ve C - -ve
Indol Test ( Kovac's Reagent): E.a. = negative = yellow/gold ring at meniscus; as for E.c. = positive = red ring at meniscus (immediate change). Methyl Red Test (Methyl Red Reagent): E.a. = negative = yellow = pH > 7.0; as for E.c. = red color = pH < 5 (immediate change). Vogues-Proskauer Test (Napthol + KOH Reagent): E.a. = positive = reddish color (not immediate change); as for E.c. = negative = brown/yellow (straw color). Citrate (Bromothymol Blue Reagent - alkaline pH): E.a. = positive = blue color + growth; as for E.c. = negative = green color.
I: + m: + v: - c: -
The IMViC series of tests provides an identification of the principal groups of Enterobacteriaceae that cause intestinal infections, by using the biochemical properties and their enzymatic reactions to specific substrates
Staphylococcus epidermidis indole test - negative methyl red - negative voges proskauer test - positive citrate test - no idea
Indole - NegativeMethyl Red - PositiveVouges Proskauer - NegativeCitrate-NegativeTSI - Alkaline slant/Acid butt with H2SSIM - Motile, H2S positiveUrease - Negative
imvic test helps in identi fication of salmonello typhi and a specific agar named salmonella shigella agar is used for culturing these species
I recently completed colony morphology on the E.colispecimen.The results displayed small colonies that were circular in shape, a diameter of approximately 0.5mm, the elevation of the colonies was seen to be slightly raised with the surface incredibly smooth.The colonies were also seen to have a pink colour pigmentation in Mac conkey agar due to its lactose fermenter after growth.The gram stain for these bacteria should be negative rod shaped.
the answer i would like to give. there are many biochemical tests for detection of various types of bacteria. 1. carbohydrate utilization tests (sugar test). - lactose , sucrose , mannitol , maltose , xylose and dextrose. 2. imvic test. it includes indole test , methyl red test , voges proskuer test , citrate utilization test. 3. nitrate reduction test. 4. urease test 5. triple sugar iron test. 6. lead acetate test. 7. mannitol motility test 8. O-F (oxidation - fermentation) test 9. aminoacid degradation test we can detect enterobacteriaecae group & other group of bacilli according to their charactristics by comparing with standard table.
The Kirby-Bauer antibiotic test is a general test that determines if an organism is resistant or susceptible to different antibiotics. Many different types of antibiotics can be used but some of the common ones are penicillin, tetracycline, chloramphenicol, and streptomycin.