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Indol Test ( Kovac's Reagent): E.a. = negative = yellow/gold ring at meniscus; as for E.c. = positive = red ring at meniscus (immediate change).

Methyl Red Test (Methyl Red Reagent): E.a. = negative = yellow = pH > 7.0; as for E.c. = red color = pH < 5 (immediate change).

Vogues-Proskauer Test (Napthol + KOH Reagent): E.a. = positive = reddish color (not immediate change); as for E.c. = negative = brown/yellow (straw color).

Citrate (Bromothymol Blue Reagent - alkaline pH): E.a. = positive = blue color + growth; as for E.c. = negative = green color.

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Q: How IMViC tests differentiate Escherichia coli from Enterobacter aerogenes?
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Indol Test ( Kovac's Reagent): E.a. = negative = yellow/gold ring at meniscus; as for E.c. = positive = red ring at meniscus (immediate change). Methyl Red Test (Methyl Red Reagent): E.a. = negative = yellow = pH &gt; 7.0; as for E.c. = red color = pH &lt; 5 (immediate change). Vogues-Proskauer Test (Napthol + KOH Reagent): E.a. = positive = reddish color (not immediate change); as for E.c. = negative = brown/yellow (straw color). Citrate (Bromothymol Blue Reagent - alkaline pH): E.a. = positive = blue color + growth; as for E.c. = negative = green color.


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