I recently completed colony morphology on the E.colispecimen.
The results displayed small colonies that were circular in shape, a diameter of approximately 0.5mm, the elevation of the colonies was seen to be slightly raised with the surface incredibly smooth.
The colonies were also seen to have a pink colour pigmentation in Mac conkey agar due to its lactose fermenter after growth.
The gram stain for these bacteria should be negative rod shaped.
E. coli forms dark blue colonies on Chromocult agar due to its ability to metabolize certain substrates present in the agar, resulting in the production of dark blue pigments such as indole. This metabolic activity is specific to E. coli and helps to differentiate it from other bacteria based on colony color.
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
* Colonies are circular, 2-3 nm in diameter with a smooth shiny surface when grown on nutrient agar,milk agar or blood agar for 24 h at 37 degrees * Colonies are often pigmented,though a few strains are unpigmented Got the answers from here http://www.geocities.com/SouthBeach/Port/3008/staphyl.html
Eosin methylene blue (EMB) agar is a growth medium that can produce red colonies of Escherichia coli. This medium contains dyes that inhibit the growth of Gram-positive bacteria and allow for the detection of lactose fermentation, resulting in the characteristic red colonies of E. coli.
Green colonies are a result of a protein called biliverdin or sometimes a variation just called hemolytic verdin. It is a result of incomplete hemolysis of blood, and is produced by any member of the pneumoniae family. If you see green bacteria on MacConkey, it is a form of penumoniae.
whitw to whitish gray
It is possible for E. coli to outcompete M. luteus on a nutrient agar if the conditions are more favorable for E. coli growth. E. coli is known for its fast growth rate and adaptability, which can give it a competitive advantage over other bacteria in certain environments. Factors such as nutrient availability and pH levels can also influence which species will dominate in a mixed culture.
The morphology of E. coli is typically rod-shaped and can vary in size. Under a microscope, E. coli appears as a single bacterial cell with a length of around 2 micrometers and a width of around 0.5 micrometers.
To grow E. coli in a petri dish, first prepare a nutrient agar medium by mixing agar with a nutrient broth, then autoclave to sterilize. Once cooled to about 50°C, pour the agar into sterile petri dishes and allow it to solidify. Inoculate the agar surface with E. coli using a sterile loop or swab, then incubate the plates upside down at 37°C for 24 hours. After incubation, observe the growth of colonies.
Bacillus
For colony appearance u want to describe what it looks like on agar not under a microscope. In terms of color it depends on what type of growth medium u are using but if it's just plain nutrient agar: Pigmentation: Colorless Opacity: Translucent Form: Circular Margin: entire Surface: Smooth Elevation: Raised.
E. coli is actually green on tryptic soy agar. The agar itself is a white, amber color.
E. coli forms dark blue colonies on Chromocult agar due to its ability to metabolize certain substrates present in the agar, resulting in the production of dark blue pigments such as indole. This metabolic activity is specific to E. coli and helps to differentiate it from other bacteria based on colony color.
E. coli will not grow on Enterococcus agar, as this medium is selective for Enterococcus species and inhibits the growth of other bacteria. Conversely, Enterococcus can grow on Endo agar, which is designed for the isolation of gram-negative bacteria like E. coli but does not specifically inhibit Enterococcus. However, the growth of Enterococcus on Endo agar may be less pronounced compared to that of E. coli.
Sheep blood agar inhibits gram negative bacteria. E. coli is gram negative.
The intended use of Nutrient Agar with MUG is for detecting an enumerating E. Coli in water. This lab wasn't looking specifically and only for e.coli. It was looking for other types of coliforms as well. this is my best guess
P. morabilis is characterized by its swarming ability. On a nutritive media plate the organism will swarm the entire plate due to its hyper mobility from its peritrichious flagella. Colonies can generally be seen to be opaque with irregular borders and have a smooth texture.