whitw to whitish gray
Yellowish
Slants are better suited than agar plates, because they can be capped, preventing the agar and the culture from drying out. The cap also prevents airborne contaminants from entering the slant. Also, slants take up less storage space than an agar plate.
Colonies growing on a pour plate have slightly less avalible oxygen and are confined by the gel matrix so they tend to grow smaller than those on a pour plate. Streak plates are use to isolate single colonies, pour plates are used to enumerate batceria.
The colonies that grew on MAC plate took up lactose from the medium for their metabolism and released an end product that caused the pH indicator of the medium (neutral red) to turn pink. Hence the colonies appears pink in color.
to see time fly! You may be referring a bacterial transformation experiment. If so, the bacteria turns color to indicate that the DNA that was transferred to the bacteria is being expressed and the transformation was successful. If you are referring to the natural color of bacteria growing in large colonies on agar, that beige color is their natural color only visible when they are growing in colonies by the millions. The yellow color could also be a fungus which has contaminated your plate which happens often in a non-sterile classroom environment. Never open a petri dish after bacterial growth or fungal growth is evident.
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
anti clock wise
Counter-clockwise
The North American plate is both shrinking and growing.
The size of the cultured colonies is usually determined by how densely the plate is populated. The more densely populated the plate, the greater the competition is among the microorganisms for nutrients. This competition results in the growth of reletively small colonies which tend to be merged together. On a more sparsely populated plate, there are enough nutrients for the microorganisms to grow sufficiently resulting in larger colonies.
How do colonies on the surface of a pour plate differ from those suspended in the agar?
Because if the plates are wet you will not get individual colonies, instead you will get a film of bacteria growing in the water film on the surface of the plate. This can ruin a selection for transformants as the antibiotic will not be present in the water film on the surface of the plate.