0
What else can I help you with?
What is the colony color of serratia on nutrient agar plate?
Yellowish
When performing a streak plate culture in what direction should one rotate the nutrient plate?
When performing a streak plate culture, the nutrient plate should be rotated in a clockwise or counterclockwise direction to create isolated colonies on different parts of the plate. The rotation ensures that the inoculating loop spreads the bacteria evenly across the agar surface, leading to distinct colonies for further analysis.
How would you record your observation of a plate containing 305 colonies?
I would describe the appearance of the plate and note the total number of colonies (305) present. It is important to record any distinct characteristics of the colonies, such as color, size, and shape, and make note of any patterns or distribution of the colonies on the plate.
Why did the colonies on the MAC plate turn pink?
The colonies that grew on MAC plate took up lactose from the medium for their metabolism and released an end product that caused the pH indicator of the medium (neutral red) to turn pink. Hence the colonies appears pink in color.
What is a streak plate?
A streak plate is a surface of unglazed ceramic, used to find the true color of a mineral specimen by drawing the specimen across it. The color of the resultant powder is referred to as the streak or streak color of a mineral.
How does the contribution of growth on a pour plate different from that on a steak plate?
Colonies growing on a pour plate have slightly less avalible oxygen and are confined by the gel matrix so they tend to grow smaller than those on a pour plate. Streak plates are use to isolate single colonies, pour plates are used to enumerate batceria.
What is the advantage of using a nutrient agar plate over a nutrient agar slant?
Slants are better suited than agar plates, because they can be capped, preventing the agar and the culture from drying out. The cap also prevents airborne contaminants from entering the slant. Also, slants take up less storage space than an agar plate.
How Cultivation of bacteria?
To cultivate bacteria, you would typically streak a sample onto a nutrient agar plate in a sterile environment. The plate is then incubated at the optimal temperature for the specific bacteria species to grow. After incubation, colonies of bacteria will form, which can be studied and analyzed.
Why did bacteria in the agar dish turn yellow?
to see time fly! You may be referring a bacterial transformation experiment. If so, the bacteria turns color to indicate that the DNA that was transferred to the bacteria is being expressed and the transformation was successful. If you are referring to the natural color of bacteria growing in large colonies on agar, that beige color is their natural color only visible when they are growing in colonies by the millions. The yellow color could also be a fungus which has contaminated your plate which happens often in a non-sterile classroom environment. Never open a petri dish after bacterial growth or fungal growth is evident.
How would you change the bacterias plate to best tell if they are ampicillin resistant?
The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.
What determines the size of cultured colonies?
The size of the cultured colonies is usually determined by how densely the plate is populated. The more densely populated the plate, the greater the competition is among the microorganisms for nutrients. This competition results in the growth of reletively small colonies which tend to be merged together. On a more sparsely populated plate, there are enough nutrients for the microorganisms to grow sufficiently resulting in larger colonies.
If you strongly suspect a nutrient agar slant culture is contaminated what method would you use to ascertain whether you are right or not?
You have to make subculture from this slant and after incubation you can observe how many types of microorganisms are present in the nutrient agar slant. If you have one colony shape so you have a pure nutrient agar slant but if you have more than one type of colonies so the nutrient agar slant is contaminated.
