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What else can I help you with?
How do you determine the number of organisms in a water sample if your dilution plate has presence of spreading colonies?
To determine the number of organisms in a water sample when spreading colonies are present on a dilution plate, you would count the number of colonies on a plate with a countable amount (usually between 30-300 colonies). Then, you would multiply this count by the dilution factor used and adjust for the volume plated to calculate the total number of organisms in the original water sample. Keep in mind that any spreading colonies need to be accounted for in this calculation.
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
What are the benefit of streak plating?
Streak plating helps isolate individual bacterial colonies on an agar plate for observation and analysis. It allows for the identification of different types of bacteria present in a sample by creating distinct and separated colonies. Streak plating is a simple and effective method for quantifying and studying bacterial populations.
Do you use in the plate or on the plate?
You would use "on the plate" when referring to placing something on top of a plate. For example, "The steak is on the plate." If referring to containing something inside a plate, you could use "in the plate," but it's less common.
Why does The hands after plate has more colonies than the hands before plate?
The hands after the plate likely have more colonies because they were exposed to various contaminants during the process of handling the plate, such as touching surfaces, utensils, or other materials that harbor bacteria and fungi. Additionally, the act of touching can transfer microorganisms from the skin to the plate, increasing the colony count. In contrast, the hands before the plate may have fewer colonies due to less exposure or more effective hygiene practices.
What one plate would you first inspect to conclude that the transformation occurred successfully Why?
If you transform bacteria with a plasmid containing a selection marker (such as an antibiotic resistance gene) and plate the transformed bacteria on a plate suited for selecting for plasmid-containing bacteria (such as a plate containing an antibiotic that only those bacteria with antibiotic resistance can survive), then simply inspecting whether colonies are present on the plate will suffice in determining whether the transformation succeeded. If no colonies are found, that means no bacteria got the antibiotic resistance gene on the plasmid and the transformation was unsuccessful. If some colonies are found, that means some bacteria contain the plamis containing the antibiotic resistance gene and those colonies can the transformation was successful.
How you differentiate between Transformed bacterial colonies and satellite colonies?
Re-streak the center of the 'star' colony (transformed surrounded by satellites) on a plate contains the antibiotic, typically ampicillin. The colonies in the tertiary streak will most likely be the transformants. If you want to be quite sure, pick a single colony from the tertiary streak and re-streak again on a plate containing the antibiotic.
How do you determine the number of organisms in a water sample if your dilution plate has presence of spreading colonies?
To determine the number of organisms in a water sample when spreading colonies are present on a dilution plate, you would count the number of colonies on a plate with a countable amount (usually between 30-300 colonies). Then, you would multiply this count by the dilution factor used and adjust for the volume plated to calculate the total number of organisms in the original water sample. Keep in mind that any spreading colonies need to be accounted for in this calculation.
Why is it important to write on the agar side of the agar plate?
It is important to write on the "Agar side" of the plate because 1. you do not want your writing on the lid to interfere with your observations and 2. If you lose the lid you won't know what you streaked (what your wrote on the lid).Hope this helps!
Why GFP colonies are observed in the plate with pQE30 and GFP construct?
Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.
How do colonies on the surface of a pour plate differ from those suspended in agar?
How do colonies on the surface of a pour plate differ from those suspended in the agar?
What are the benefit of streak plating?
Streak plating helps isolate individual bacterial colonies on an agar plate for observation and analysis. It allows for the identification of different types of bacteria present in a sample by creating distinct and separated colonies. Streak plating is a simple and effective method for quantifying and studying bacterial populations.
Which method often results in colonies developing down throughout the agar and some colonies on the surface?
The pour plate method often results in colonies developing both down throughout the agar and on the surface. This is because the pour plate involves mixing the bacteria with the agar before pouring it into the plate, allowing for colonies to form at different depths within the agar.
Do you use in the plate or on the plate?
You would use "on the plate" when referring to placing something on top of a plate. For example, "The steak is on the plate." If referring to containing something inside a plate, you could use "in the plate," but it's less common.
Can you tell if these bcteria are ampicillin resistant by looking at them on the LB plate?
No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured, think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.
What is an assiette?
An assiette is a plate of food, a small plate containing the same food item prepared in different ways.
How to determine Bacterial load?
By pour plate and then counting the colonies.
