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No. You cannot tell if the bacteria are ampicillin resistant just by looking at them. Both types of bacteria (those that are ampicillin resistant and those that are ampicillin sensitive) look similar when cultured, think about the colonies on the LB starter plate and the colonies on the +pGLO LB/amp plate.

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What science equipment would be best to use during an investigation about evaporation where a hot plate is being used to evaporate water?

A balance for measuring the mass of the water before and after evaporation, a thermometer to monitor the temperature of the water as it evaporates, and a timer to track the duration of evaporation. Safety equipment like goggles and heat-resistant gloves are also essential when using a hot plate.


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Related Questions

How would you change the bacterias plate to best tell if they are ampicillin resistant?

The best test would be to take some of the bacteria growing on the LB plate and streak them on a LB/amp plate. If the bacteria are viable on the LB/amp plate, then they are resistant to ampicillin. If no bacterial colonies survive, then they were not ampicillin resistant.


What if a agar plate is isolated and antibiotic added and result is no growth or inhibition?

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Behrenberg Company makes a shatter resistance plate set.


What is meant by a control plate in pglo experiments?

a control plate in your particular case is a ampicillin plate with no inoculation of your transformed bacteria. its purpose to make sure that during the process of handling the plate there were no contamination by bacteria in the surrounding.


Why GFP colonies are observed in the plate with pQE30 and GFP construct?

Go to http://www.tsienlab.ucsd.edu/Images.htm you will find bacterial colonies will and can express functional fluorescent proteins. As for the plasmid pQE30, it looks to fit into the category of expression at this level.


On which plate would you expect to find bacteria most like the original non transformed E. coli colonies you initially observed?

You would expect to find it in the plate labeled LB-, because that specific plate is the control plate, meaning it has nothing added to it, like the amipicilin or pFlouroGreen plasmid.


Which plates should be compared to prove that the changes that occurred were due to the procedure that you performed?

The best way to prove that these changes occurred in the transformation lab is to compare the control to the experimental plates. Cells that were not treated with the plasmit (LB/amp (-) pGLO and LB/amp/are (-) pGLO plates) could not grow on ampicillin, wheras cells that were treated with the plasmid (LB/amp (=) pGLO and lB/amp/ara (+) pGLO plate) can grow on the LB/amp plate. Thus, the plasmid must confer resistance to ampicillin.


How do antibiotic resistant bacteria come about?

This term is misleading. The antibiotic "selects" bacteria that are not affected by it. If a person will grow bacteria on a petri dish and add an antibiotic to it, some bacteria may live and grow. This is actually a form of natural selection. The ones that will grow are resistance to the antibiotic. They have some way of not being affected. If a person takes a colony from the plate that has this resistance and grows it on another plate and add the antibiotic, all on the plate will be resistant.


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Abrasion resistant steel plates, also known as wear resistant steel plates, are widely used in areas wear the wear and tear of plates is high, such as mining equipment, truck tippers, and cement industry. For more information on the usage of abrasion resistant steel plates, see the related link.


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