to see time fly!
You may be referring a bacterial transformation experiment. If so, the bacteria turns color to indicate that the DNA that was transferred to the bacteria is being expressed and the transformation was successful. If you are referring to the natural color of bacteria growing in large colonies on agar, that beige color is their natural color only visible when they are growing in colonies by the millions.
The yellow color could also be a fungus which has contaminated your plate which happens often in a non-sterile classroom environment.
Never open a petri dish after bacterial growth or fungal growth is evident.
Well it depends on which type of agar preparation you use. For example, if you use a mannitol NaCl salt agar it'll contain a red phenol dye which is a pH indicator. If a microbe is pathogenic it'll ferment the mannitol into acids which turn the red indicator yellow. Non-pathogenic bacteria such as S. epidermidis will not ferment the mannitol and so no colour change will result.
That really depends on what agar you are growing them on. If you are using growing agars such as BHI or blood, both will grow, and you won't be able to tell the difference when looking at them on the plate. If you use a selective agar that, lets say, will only grow cocci, then the bacilli won't grow. Or vice versa, if your selective agar only grows bacilli, then the cocci won't grow. If you use a differential agar, it may turn one a different color than the other -- for example, it may turn the bacillus blue, and the cocci may turn red. It is all dependent on the media you choose to grow your samples.
The limewater would likely disrupt the pH balance inside the bacterial cells, affecting their ability to function properly. This could lead to cell damage or death depending on the concentration of limewater and the type of bacteria present in the dish.
Serratia marcescens and some strains of Chromobacterium violaceum are known to produce red pigments at room temperature. These pigments are sometimes visible in agar cultures as a red color.
Mannitol salt agar supports growth of organisms that can grow in a high salt concentration, particularly Staphylococcus species and halophiles. The phenol red pH indicator in the agar will also let you know whether or not the bacterium you streaked ferments mannitol by changing to a yellow color if fermentation has occured.
Well it depends on which type of agar preparation you use. For example, if you use a mannitol NaCl salt agar it'll contain a red phenol dye which is a pH indicator. If a microbe is pathogenic it'll ferment the mannitol into acids which turn the red indicator yellow. Non-pathogenic bacteria such as S. epidermidis will not ferment the mannitol and so no colour change will result.
Macconkey agar consists of agar, lactose, peptone, neutral red dye, crystal violet dye & bile salts. Bile salts inhibit growth of Gram positive bacteria & allow growth of Gram negative bacteria only. It differentiates lactose fermentating Gram negative bacteria from non lactose fermentating Gram negative bacteria.
That really depends on what agar you are growing them on. If you are using growing agars such as BHI or blood, both will grow, and you won't be able to tell the difference when looking at them on the plate. If you use a selective agar that, lets say, will only grow cocci, then the bacilli won't grow. Or vice versa, if your selective agar only grows bacilli, then the cocci won't grow. If you use a differential agar, it may turn one a different color than the other -- for example, it may turn the bacillus blue, and the cocci may turn red. It is all dependent on the media you choose to grow your samples.
it could have been contaminated.
The membrane filter / agar plates produce colonies from bacteria that can grow on the agar you are using (not all fecal coliforms can). The Colilert will turn color if the bacteria have the enzyme to metabolize substrates in the Colilert (not all do). If all fecal coliform bacteria could grow on the agar and all fecal Coliforms had the right enzymes you'd get the same numbers.
When grown on crystal violet agar, E. coli typically exhibits a purple color. This is due to the retention of the crystal violet dye by the bacterial cells, which is a characteristic reaction for Gram-negative bacteria when subjected to this type of medium. The purple color indicates the presence of the bacteria, as the dye binds to the cell wall components.
If you added more glucose that the Kliger Iron Agar test than is called for you risk getting false results. The bacteria need to exhaust the glucose to turn the solution red or yellow. If too much glucose is present you may not be able to determine if the bacteria can ferment animal proteins.
The limewater would likely disrupt the pH balance inside the bacterial cells, affecting their ability to function properly. This could lead to cell damage or death depending on the concentration of limewater and the type of bacteria present in the dish.
Any disease that damages your liver or kidneys can turn you yellow (jaundice). Flesh eating bacteria and serious infections can turn you purple. Normal infections can create a reddish appearance.
Could be iron bacteria. Could also be the adhesive. We have yellow stains on our roll flooring from the installer using the wrong adhesive. Tom
They probably can't turn yellow, but some of them are naturally yellow..........
Well, Macconkey Agar II plates have Gram Crystal Violet in them so there are a number of bacterial species that can absorb it and turn "very pink." The way to narrow it down is to identify whether it grows in chains or clusters. But other than that, it is hard to tell.