0
The use of silica gel can be interpreted in two ways. It well know that silica (SiO2) can adsorb water on its surface. If you use silica dryed under 400 °C, the process of adsorption occurs by the silanol group. So, molecules with a polar groups like hydroxil, amine, nitrilic ... can make chemical interactions with the silica surface and can be retained more time into a column. In this case the adsorption is chemical. But, if you use a deactived silica (~5% H2O), the H2O molecules make a layer into a silica surface and the main process occurs by partition chromatography.
Wiki User
∙ 11y ago
Add your answer:
Earn +20 pts
How high-performace liquid chromatography works?
High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.
What are some possible alternatives for paper chromatography?
Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works.
What pen works best on chromatography?
Dark colors like black and brown.
How can chromatography separate out the different pigments in the paint?
Chromatography works by separating inks into the different colours they are made of. You see, the paint is a solute and if it is added to a solvent and dissolves you have a solution.
How is chromatography a analytical tool?
Chromatography is an analytical tool used to separate and analyze complex mixtures. It works based on the principle that different components in a mixture will move at different rates through a stationary phase when subjected to a mobile phase. By analyzing the resulting separation pattern, chromatography can provide valuable information about the composition and identity of the mixture being analyzed.
Which property of compounds is responsible for the separation in TLC?
The general Principle involved in tlc is similar to that of column chromatography i.e. adsorption chromatography. In the adsorption process, the solute competes with the solvent for the surface sites of the adsorbent. Depending on the distribution coefficients, the compounds are distributed on the surface of the adsorbent.
How does chiral Hplc work?
As a very crude analogy, think of a playground slide with dents in it that are the size and shape of a left shoe print. If we put some shoes at the top and let them slide down, the left shoes will be more likely to get stuck in the dents than the right shoes (which don't fit as well and therefore slide over them more easily).
How high-performace liquid chromatography works?
High-performance liquid chromatography (HPLC) works by pumping a liquid sample through a column packed with tiny particles. These particles have different affinities for the components of the sample, causing them to separate as they pass through the column. The separated components are then detected by a detector, which produces a chromatogram. HPLC is commonly used in analytical chemistry to separate and quantify compounds in a mixture.
What are some possible alternatives for paper chromatography?
Coffee filter paper can be used. It does not produce results as good as chromatography paper, but it still works.
What pen works best on chromatography?
Dark colors like black and brown.
How can chromatography separate out the different pigments in the paint?
Chromatography works by separating inks into the different colours they are made of. You see, the paint is a solute and if it is added to a solvent and dissolves you have a solution.
How dry out silicon crystals?
I think you can dry silicon crystals by microwave. I know it works with silica gell.
How is chromatography a analytical tool?
Chromatography is an analytical tool used to separate and analyze complex mixtures. It works based on the principle that different components in a mixture will move at different rates through a stationary phase when subjected to a mobile phase. By analyzing the resulting separation pattern, chromatography can provide valuable information about the composition and identity of the mixture being analyzed.
What colours could have no effect in the process of chromatography?
This question is not very precise, but I assume you mean which colours will not be separated by chromatography. It is not a property of the colour, but of the substance you are trying to split up. Chromatography only works if the substance is soluble in the liquid you are using to run the chromatogram. Thus some black fountain pen inks separate in water, but the ink from a ball point pen usually does not.
What pen works best with chromatography?
mooo! Black ink pen mooo!! *makes noises in backround mooo!* and any other dark ink will work too
Is basalt have silicon in it?
Yes. All igneous rocks contain silicon. By weight basalt is 45-53% silica, which works out to 21-24% silicon.
How does hplc works?
HPLC works when a reservoir holds the solvent and then it is sent to the pump manager.Next it goes to the HPLC coloumn .After it goes through there it usually ends in the detector than waste. Generally the stationary phase in the HPLC column is made up of alkyl coated silica making it relatively non-polar. Due to this the technique is also called reversed-phase HPLC.
