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Which property of compounds is responsible for the separation in TLC?
Wiki User
∙ 15y ago
The general Principle involved in tlc is similar to that of column chromatography i.e. adsorption chromatography. In the adsorption process, the solute competes with the solvent for the surface sites of the adsorbent. Depending on the distribution coefficients, the compounds are distributed on the surface of the adsorbent.
Wiki User
∙ 11y ago
Wiki User
∙ 15y agoChromatography works by differences in polarity.
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Why do some compounds travel further on TLC plates than others?
it doesn't depends upon the TLC plates.. as it depends upon the solvents in which these compounds migrate.
How does TLC help to determine the identity and purity of compounds?
By separating the compounds into separate dots on the plate. It is pure if the only dot showing up is that of the tested product
What are the TLC Rf values influencing factors?
Response Details:1) factor that affects the Rf value of TLC is the polarity of the solvent. If the solvent is too polar all of the compounds can run right up the plate resulting in poor separation.2) The RF value is used to established the identity of the spots of TLC plate.3) The RF value is ratio of the distance the solvent travels to the distance compound travels.4) The factors can affect RF values. The absorbent uniformity on the thin layer plate, same concentration(spotting is too weak or strong), room temperature during the mobile phase and development distance of the solvent during the mobile phase can all affect the results.5) Multiple solvent system are necessory when utilizing TLC for identification purposes.Response Details:1) factor that affects the Rf value of TLC is the polarity of the solvent. If the solvent is too polar all of the compounds can run right up the plate resulting in poor separation.2) The RF value is used to established the identity of the spots of TLC plate.3) The RF value is ratio of the distance the solvent travels to the distance compound travels.4) The factors can affect RF values. The absorbent uniformity on the thin layer plate, same concentration(spotting is too weak or strong), room temperature during the mobile phase and development distance of the solvent during the mobile phase can all affect the results.5) Multiple solvent system are necessory when utilizing TLC for identification purposes.
What is difference between HPLC and TLC?
TLC= In it finely solid is spread on a rigid supporting plate (stationary phase)and the mobile phase is allowed to migrate across the surface of plate by capillary action. Less efficient then HPLCHPLC= in it column are used on place of plates and the mode of separation is adsorption or partition coefficient
Why is TLC a poor technique for the analysis of low boiling liquids?
TLC is a slow technique so during the process the low boiling liquids become evaporated an we can't observe their presence.
What will be the result of applying too much compound to a TLC plate?
Applying too much compound to the TLC plate makes it difficult for a good separation to occur. Instead, a large blot of solution tends to appear in the TLC. "It is extremely important that the spots be as small as possible and that they be applied using a 1% solution of the compounds being separated
Why do some compounds travel further on TLC plates than others?
it doesn't depends upon the TLC plates.. as it depends upon the solvents in which these compounds migrate.
Why is it important to use a small amount of sample when spotting a TLC sheet?
Spot small amount of sample so that it run as a discrete spot and doesn't diffuse out into a large area. It make the separation from other compounds much more efficient.
Arrange the following compounds in order of increasing Rf values in TLC Anthracene benzil tryphenylmethanol?
The order of increasing RF values in TLC is Benzil, methanol anthracene and tryphenyl.
How does TLC help to determine the identity and purity of compounds?
By separating the compounds into separate dots on the plate. It is pure if the only dot showing up is that of the tested product
Is a TLC Plate the same as filter paper?
No. A TLC (Thin Liquid Chromatagraphy) plate is made specially. It has different Compounds to it that make it separate from filter paper. See 1st related link below for more info on TLC Plates See 2nd related link for info about filter paper
How is chromatography used to identify individual compounds?
Thin layer chromatography can be used to identify a compound because for certain reaction mixtures, TLC can separate all compounds in a mixture based on the distance the compound travels on the TLC film. With the proper solvent system, TLC can be used to uniquely identify a compound.
What are the TLC Rf values influencing factors?
Response Details:1) factor that affects the Rf value of TLC is the polarity of the solvent. If the solvent is too polar all of the compounds can run right up the plate resulting in poor separation.2) The RF value is used to established the identity of the spots of TLC plate.3) The RF value is ratio of the distance the solvent travels to the distance compound travels.4) The factors can affect RF values. The absorbent uniformity on the thin layer plate, same concentration(spotting is too weak or strong), room temperature during the mobile phase and development distance of the solvent during the mobile phase can all affect the results.5) Multiple solvent system are necessory when utilizing TLC for identification purposes.Response Details:1) factor that affects the Rf value of TLC is the polarity of the solvent. If the solvent is too polar all of the compounds can run right up the plate resulting in poor separation.2) The RF value is used to established the identity of the spots of TLC plate.3) The RF value is ratio of the distance the solvent travels to the distance compound travels.4) The factors can affect RF values. The absorbent uniformity on the thin layer plate, same concentration(spotting is too weak or strong), room temperature during the mobile phase and development distance of the solvent during the mobile phase can all affect the results.5) Multiple solvent system are necessory when utilizing TLC for identification purposes.
How did the Duggar family earn enough to afford their children before TLC?
I think they owned property or buildings that people paid rent for.
What is difference between HPLC and TLC?
TLC= In it finely solid is spread on a rigid supporting plate (stationary phase)and the mobile phase is allowed to migrate across the surface of plate by capillary action. Less efficient then HPLCHPLC= in it column are used on place of plates and the mode of separation is adsorption or partition coefficient
What is the name of the method used to separate dyes?
Chromatography is the method used to separate a mixture of dyes or inks.
Was Beyonce ever in TLC?
No, Beyonce was never apart of the group TLC. She was in Destiny's child but never TLC
