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How do tandemly arranged repeats affect the lengths of restriction fragments?
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Why are restriction enzymes necessary in the DNA fingerprinting process?
because DNA is the process of getting heriderity informationAns2:Restriction enzymes clip the DNA strand and create short fragments that can be processed. If you clip the strand at a known combination, you will know that every resulting fragment ends with that combination. Knowing the lengths of the fragments allows you to identify where that combination would be located on the complete strand.
What does the charge of DNA have to do with DNA fingerprinting?
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
Why does extracted DNA need to be fragmented?
In preparation for the electrophoresis step in "DNA fingerprinting" the electrophoresis process cannot separate meaningfully massive molecules like whole chromosomes. By using restriction enzymes that break the chromosomes at known places DNA fragments of a wide variety of lengths that the electrophoresis process can separate meaningfully will allow a pattern to be generated that can identify different individuals.
How are restriction maps used?
They are used to show the lengths of DNA fragments between restriction sites in a strand of DNA.
How do restriction sites and a restriction map relate?
Restriction maps show the lengths of DNA fragments between restriction sites in a strand of DNA.
Why are restriction enzymes necessary in the DNA fingerprinting process?
because DNA is the process of getting heriderity informationAns2:Restriction enzymes clip the DNA strand and create short fragments that can be processed. If you clip the strand at a known combination, you will know that every resulting fragment ends with that combination. Knowing the lengths of the fragments allows you to identify where that combination would be located on the complete strand.
What determines the distance of the DNA fragments?
By the size of the DNA molecule. longer DNA molecules move slower, and shorter DNA molecules move faster!
What does the charge of DNA have to do with DNA fingerprinting?
DNA is of a negative charge. So when gel electrophoresis is used on it the DNA fragments are attracted to the positive end of the electrophoresis. The fragments of different lengths travel down the gel towards this end. The longer length fragments travel less and so are farther from the positive end. By looking at these DNA fragments, which are created by cutting DNA with restriction enzymes one can compare and contrast DNA. Thus DNA fingerprinting can take place based on the different restriction sites in DNA (cut by the enzymes) forming different length segments of DNA.
Differences in DNA sequence on homologous chromosomes that can result in different patterns of restriction fragment lengths?
Restriction fragment length polymorphism (RFLP)
How are RFLP made?
A DNA sample is broken into pieces by restriction enzymes and the resulting fragments are separated according to their lengths by gel electrophoresis. RFLP analysis was the first DNA profiling technique inexpensive enough to see widespread application. But isn't as widely used now.
Explain how an agarose gel can separate DNA fragments of different lengths.?
The separation of DNA fragments is based on size. When a DNA sample is run in a gel (electrophoresis), the lighter fragments migrate faster than the heavier (longer) fragments under the influence of an electric current. At the and of the process, the shorter fragments are found at the terminal end of the gel and the longer fragments closer to the origin
What are the consecutive sides of a quadrilateral?
The only thing that can be said about consecutive sides of a quadrilateral is that they meet at a vertex. There is no restriction on the angle, nor on their respective lengths.
Why does extracted DNA need to be fragmented?
In preparation for the electrophoresis step in "DNA fingerprinting" the electrophoresis process cannot separate meaningfully massive molecules like whole chromosomes. By using restriction enzymes that break the chromosomes at known places DNA fragments of a wide variety of lengths that the electrophoresis process can separate meaningfully will allow a pattern to be generated that can identify different individuals.
Do dominant alleles move farther in electrophoresis?
Not always. Different chromosomal fragments travel different distances in electrophoresis due to their different lengths. Longer fragments are heavier and therefore travel shorter distances under the same electrical force.
Why would other bands be present in gel electrophoresis if they are not supposed to be present?
This answer assumes that prior to the electrophoresis, you have applied a restriction enzyme to the DNA which breaks it up into fragments of different lengths. Electrophoresis separates fragments of DNA according to their molecular mass, size and charge. Each band will represent a pool of fragments that are the same length. The shortest, lightest fragments will travel the furthest through the gel, where as the long, heavy fragments will not travel very far. The darkness of the band also indicates the frequency of that particular length fragment.
