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Barbituric acid looks like its very polar... it should be soluble in water and acetone, at least.

Visualizing it on a TLC plate is problematic because the compound is not aromatic and thus does not absorb UV. The easiest way to visualize non-UV absorbing compounds on a TLC plate is using an iodine development chamber. In a jar, mix some powdered iodine (I2) crystals* and dry silica gel. Throw in the TLC plate (after running in the appropriate solvent system), shake it a bit, and use forceps to fish it out. The iodine should oxidize the compound on the plate and it should appear as a brown dot. The iodine development chamber can be used pretty much indefinitely. Hope this helps!

  • Do all steps in the fume hood... pure iodine is toxic.
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Q: How do you check TLC for thio barbituric acid it wont soluble in any solvent?
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