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Ideally, the void volume should be 40% of the total column volume.
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∙ 11y ago
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What is void volume in chromatography?
bed volume= pie*r square*h where, pie=3.14 r=radius of column h=height of column it is the total volume of column packed with the gel.
What is void volume in hplc?
Total volume of mobile phase in a fully wet packed column- the space between the particles of the stationary phase plus the volume within the particles
What is void rate?
Void ratio is defined as the ratio of the volume of the void space compared to the volume of the solid particles.
How can you calculate the volume of void space of sand?
What fraction of the soil volume is void space
How do you calculate column volume?
Multiply column inside diameter by the column's length. Then convert to units you need. The above is not correct.The volume of a column is the circular area of the column multiplied by the length, pi*radius^2*length.
Can light weight cement be used to fill void in brick column?
yes
What is hplc column volume?
Since an HPLC column is a cylinder, the simplest estimate for the column volume is the equation V=L*pi*r2, where L = length of column (typically 50-250 mm, or 5-25 cm), and r=radius of the column, where typical internal diameters of HPLC columns are 2.1 mm, 3 mm, 4 mm, and 4.6 mm. For example, suppose you have a column that is 25 cm long by 4.6 mm internal diameter (ID). Since the ID is in mm, you first convert to cm, then divide by 2 to get 0.23 cm radius. The column volume equation then is: V = 25 * pi * (0.23)2 = 25 * pi * 0.0529 = 1.3225 * pi = 4.15 cm3 From there, you can convert cm3 to mL directly, so your column has a volume of 4.15 mL. However, you must also allow for the relative porosity of the packing material in your column, which is harder to measure. Typically, an unretained analyte will be injected through the column at a known flow rate, and the time it takes for the analyte to exit the column is used to determine a better approximation of column volume. In the case of using an unretained analyte (which in reversed-phase HPLC, the analyte might be Uracil), using the same 25 cm by 4.6 mm column above and a 1 mL/min flow rate, suppose the analyte elutes from the column at 3.2 minutes. The column volume would then be 3.2 minutes * 1.0 mL/min = 3.2 mL, which does not agree with the calculated column volume. This is due to the fact that the particles in the column take up some of the volume of the column, so the total column volume is reduced by the amount of space they take up.
What is the formula for void ratio?
Volume of voids divided by Volume of solids
What would you do to determine the volume of a rock?
You would determine the volume of water it displaces and measure it.
Is it harder to determine mass or volume?
volume
Calculate area of a round column?
How to calculate round column volume. +== No formula given so how can the "answer" be useful? The volume of a round column of radius r and height h is that of any cylinder: r^2.pi.h.
How do you determine volume?
Penis
