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Determine the population of bacteria based on the bacterial colonies?
Assume each colony started as a single bacteria in the original culture. Count the colonies you have and multiply up according to how diluted you made the culture and how much of the original culture you used.
What are the advantages of serial dilution of an agar plate?
Serial dilution of an agar plate allows for the quantification of bacterial colonies by providing a range of colony counts within the plate, making it easier to count without overcrowding. It also helps to isolate individual colonies for further analysis or microbiological testing. Additionally, serial dilution can help determine the original concentration of bacteria in a sample by calculating the dilution factor.
What is the type of plating technique that is probably performed when using the above dilution technique?
The plating technique most likely performed when using the dilution technique is spread plating. In spread plating, a sample is spread over the surface of the agar plate using a sterile spreading tool to obtain individual colonies. This method helps to isolate and quantify bacteria present in the sample.
Why dilution is a common approach employed by any pure culture technique?
the main purpose of this is to grow and isolate all bacteria present in an infection, to determine which of the bacteria that grew are most likely causing the infection and which are likely colonizers.
How do you Calculate the bacteria per ml present in a contaminated urine specimen?
To calculate the bacteria per milliliter (ml) in a contaminated urine specimen, first, perform a quantitative culture by diluting the urine sample and plating it on an appropriate agar medium. After incubation, count the number of colonies that grow on the plate. Multiply the number of colonies by the dilution factor and the volume of the plated sample (in ml) to determine the concentration of bacteria per ml in the original urine specimen. For example, if you plated 0.1 ml of a 1:100 dilution and counted 50 colonies, the calculation would be 50 colonies × 100 (dilution factor) × 10 (to convert 0.1 ml to 1 ml), resulting in 50,000 bacteria/ml.
Determine the population of bacteria based on the bacterial colonies?
Assume each colony started as a single bacteria in the original culture. Count the colonies you have and multiply up according to how diluted you made the culture and how much of the original culture you used.
What are the advantages of serial dilution of an agar plate?
Serial dilution of an agar plate allows for the quantification of bacterial colonies by providing a range of colony counts within the plate, making it easier to count without overcrowding. It also helps to isolate individual colonies for further analysis or microbiological testing. Additionally, serial dilution can help determine the original concentration of bacteria in a sample by calculating the dilution factor.
What if your instructor asks you to determine the number of organisms in a water sample observation of your dilution plates reveals?
I think you would eliminate plate counts that are not between 30-300 colonies. <30, because its too few to count, >300 too numerous to count.
Spreading 50 microL of tank water with the dilution from 10-3 to 10-5 incubation there were no colonies in any of the plates how should you record the total viable cells in cfuml of?
Some laboratories use different methods in counting colonies. In the lab I work in, we would call that TFTC or Too Few To Count.
Common mistake in dilution streak?
One common mistake in dilution streaking is applying too much pressure on the loop while streaking, resulting in overlapping of bacteria and inaccurate dilution. This can lead to incorrect colony counts and difficulty in isolating single colonies. It is important to maintain a light touch on the agar surface to achieve proper dilution and clear separation of colonies.
What is the type of plating technique that is probably performed when using the above dilution technique?
The plating technique most likely performed when using the dilution technique is spread plating. In spread plating, a sample is spread over the surface of the agar plate using a sterile spreading tool to obtain individual colonies. This method helps to isolate and quantify bacteria present in the sample.
How can one determine the concentration after dilution?
To determine the concentration after dilution, use the formula: C1V1 C2V2. C1 is the initial concentration, V1 is the initial volume, C2 is the final concentration, and V2 is the final volume. Simply plug in the values and solve for C2 to find the concentration after dilution.
Why dilution is a common approach employed by any pure culture technique?
the main purpose of this is to grow and isolate all bacteria present in an infection, to determine which of the bacteria that grew are most likely causing the infection and which are likely colonizers.
How do you Calculate the bacteria per ml present in a contaminated urine specimen?
To calculate the bacteria per milliliter (ml) in a contaminated urine specimen, first, perform a quantitative culture by diluting the urine sample and plating it on an appropriate agar medium. After incubation, count the number of colonies that grow on the plate. Multiply the number of colonies by the dilution factor and the volume of the plated sample (in ml) to determine the concentration of bacteria per ml in the original urine specimen. For example, if you plated 0.1 ml of a 1:100 dilution and counted 50 colonies, the calculation would be 50 colonies × 100 (dilution factor) × 10 (to convert 0.1 ml to 1 ml), resulting in 50,000 bacteria/ml.
How can one determine the dilution concentration of a solution?
To determine the dilution concentration of a solution, you can use the formula: C1V1 C2V2. This formula relates the initial concentration (C1) and volume (V1) of the original solution to the final concentration (C2) and volume (V2) of the diluted solution. By rearranging the formula and plugging in the known values, you can calculate the dilution concentration of the solution.
What is the formula used for counting CFU ml?
cfu/ml = (no. of colonies x dilution factor) / volume inoculated
What is a Dilution Test?
A dilution test is a procedure used to measure the concentration of a substance in a solution by systematically diluting the solution and observing the impact on the concentration. This test helps to determine the original concentration of the substance by comparing it with the concentration after dilution.
