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To determine the number of organisms in a water sample choose a plate that has less than 300 but more than 30 colonies. Then count the number of colonies and record the results.

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Q: How do you determine the number of organisms in a water sample if your dilution plate has presence of spreading colonies?
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What if your instructor asks you to determine the number of organisms in a water sample observation of your dilution plates reveals?

I think you would eliminate plate counts that are not between 30-300 colonies. <30, because its too few to count, >300 too numerous to count.


Why dilution is a common approach employed by any pure culture technique?

the main purpose of this is to grow and isolate all bacteria present in an infection, to determine which of the bacteria that grew are most likely causing the infection and which are likely colonizers.


Spreading 50 microL of tank water with the dilution from 10-3 to 10-5 incubation there were no colonies in any of the plates how should you record the total viable cells in cfuml of?

Some laboratories use different methods in counting colonies. In the lab I work in, we would call that TFTC or Too Few To Count.


What is the formula used for counting CFU ml?

cfu/ml = (no. of colonies x dilution factor) / volume inoculated


Why the sample need dilution?

Dilution is done to decrease the amount of colonies found in the plates. If the solution is not diluted, it would be difficult to count the number of colonies because they would all overlap one another. So by dilution, we can limit the amount of colonies overlapping each other, count them, and used this number to estimate the number of bacteria in the original sample.


How isotope dilution is used to determine blood volumes in living animals?

Isotope dilution is used to determine blood volumes in living animals by predicting their isotope numbers. An example is the radioactive isotopes have a 1/2 life and that would mean it goes down by half each time.


Why is dilutions a necessary part of pure culture preparation?

Dilution is necessary for getting isolated colonies, from which pure culture shall be obtained for further study.


What is the effect of dilution on viscosity of oil?

dilution will reduce the viscosity The effect of dilution on viscosity of oil is that it will decrease.


What sentence has the word dilution in it?

yummy dilution water


What does 0 percent dilution mean?

Means that there is no dilution.


Definition of dilution and dilution factor?

In chemistry and biology, the dilution factor is the total number of unit volumes in which the material is dissolved. As I understand it, the dilution refers to the dilution ratio. If you add 1 part of something to 4 parts of something else, the dilution ratio is 1 to 4. The dilution factor counts all the parts and expresses the same thing as 1 out of 5.


Is dispersement a word?

Yes, it is. A dispersement is a spreading out, dilution, a thinning. BP is currently trying to disperse the oil spill in the Gulf of Mexico, a process described as a dispersement.Please do not confuse with disbursement, which is a paying out of money or value.