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Make solutions of Citric Acid (A) and Dibasic Sodium Hydrogen Phosphate (B) according to your desired buffernig strength where A is half the concentration of B, e.g. 0.1M A and 0.2M B

To prepare the buffer take the amount of solution B (in ml) listed in the table below and fill it up to 100 ml using solution A.

pH ,0 ,1 ,2 ,3 ,4,5 ,6 ,7 ,8 ,9 2 10,8 13,2 15,6 18,1 3 20,6 22,6 24,7 26,6 28,5 30,3 32,2 33,9 35,5 37,1 4 38,6 40,0 41,4 42,7 44,0 45,4 46,7 48,0 49,3 50,4 5 51,5 52,6 53,6 54,7 55,8 56,9 58,0 59,2 60,5 61,8 6 63,2 64,6 66,1 67,7 69,3 71,0 72,8 74,8 77,2 79,8 7 82,4 85,6 86,9 88,8 90,7 92,1 93,6 94,6 95,7

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14y ago
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Q: How do you make citrate-phosphate buffer?
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